NecroIr1
Based on 1 Customer Validation
NecroIr1 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr1 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr1 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and Mixed Lineage Kinase (MLKL), and regulates CDK4 expression.
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- Pureza: 98.53%
- Fòrmula: C40H29ClIrN5O-
- Peso molecular:823.36
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
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CDK4 |
RIPK3 |
RIPK1 |
NecroIr1 (2 μM; 1-2 d) exerts subcellular distribution over 90% accumulated in mitochondria in non-labelled A549R and CFSElabelled L02 cells[1].
NecroIr1 (1.5 μM and 3 μM; 24 h) results ROS generation increasing and loss of mitochondrial membrane potential[1].
NecroIr1 (1.5 μM and 3 μM; 24 h) activates necroptosis proteins, increases the phosphorylation of RIPK1 and RIPK3[1].
NecroIr1 (0.75 μM and 1.5 μM; 24 h) induces necroptosis by arresting cell cycle at G0/G1[1].
NecroIr1 (0.75-3.0 μM; 24 h) inhibits A549R cells proliferation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549R cells
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Concentration:1.5 μM and 3 μM
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Incubation Time:24 hours
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Result:Increased phospho-RIPK1 (p-PIPK1), total RIPK3, and phospho-RIPK3 (p-PIPK3) level.
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Cell Line:A549R cells
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Concentration:0 μM, 0.75 μM and 1.5 μM
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Incubation Time:24 hours
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Result:Arrested cell cycle at G0/G1 phase in a dose-dependent manner.
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Cell Line:A549R cells
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Concentration:0 μM, 0.75 μM, 1.5 μM and 3.0 μM
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Incubation Time:24 hours
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Result:Inhibits cell proliferation in a dose-dependent manner.
Chemical Information
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Appearance Solid
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Peso molecular 823.36
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Fòrmula C40H29ClIrN5O-
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Color Orange to red
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SMILES
CC1=CC(C2=[N]3C=CC(C4=NC(C=CC=C5)=C5O4)=C2)=[N]([Ir]67([N]8=CC=CC=C8C9=C7C=CC=C9)3[N]%10=CC=CC=C%10C%11=C6C=CC=C%11)C=C1.[Cl-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
DMSO : 100 mg/mL (121.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2145 mL | 6.0727 mL | 12.1454 mL | 30.3634 mL |
| 5 mM | 0.2429 mL | 1.2145 mL | 2.4291 mL | 6.0727 mL | |
| 10 mM | 0.1215 mL | 0.6073 mL | 1.2145 mL | 3.0363 mL | |
| 15 mM | 0.0810 mL | 0.4048 mL | 0.8097 mL | 2.0242 mL | |
| 20 mM | 0.0607 mL | 0.3036 mL | 0.6073 mL | 1.5182 mL | |
| 25 mM | 0.0486 mL | 0.2429 mL | 0.4858 mL | 1.2145 mL | |
| 30 mM | 0.0405 mL | 0.2024 mL | 0.4048 mL | 1.0121 mL | |
| 40 mM | 0.0304 mL | 0.1518 mL | 0.3036 mL | 0.7591 mL | |
| 50 mM | 0.0243 mL | 0.1215 mL | 0.2429 mL | 0.6073 mL | |
| 60 mM | 0.0202 mL | 0.1012 mL | 0.2024 mL | 0.5061 mL | |
| 80 mM | 0.0152 mL | 0.0759 mL | 0.1518 mL | 0.3795 mL | |
| 100 mM | 0.0121 mL | 0.0607 mL | 0.1215 mL | 0.3036 mL |
NecroIr1 Related Classifications
HY-148365 Related Classifications
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