2. Metabolic Enzyme/Protease
  3. Angiotensin-converting Enzyme (ACE)
  4. H-Tyr-Tyr-OH

H-Tyr-Tyr-OH  (Synonyms: L-Tyrosyl-L-tyrosine)

Cat. No.: HY-114782 Purity: ≥97.0%
COA Handling Instructions

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H-Tyr-Tyr-OH (L-Tyrosyl-L-tyrosine) is an antihypertensive peptide. H-Tyr-Tyr-OH inhibits angiotensin I-converting enzyme (ACE) with an IC50 value of 0.028 mg/mL. H-Tyr-Tyr-OH can be used for the research of high blood pressure.

For research use only. We do not sell to patients.

H-Tyr-Tyr-OH Chemical Structure

H-Tyr-Tyr-OH Chemical Structure

CAS No. : 1050-28-8

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Based on 1 publication(s) in Google Scholar

H-Tyr-Tyr-OH Related Antibodies

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  • Purity & Documentation

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Description

H-Tyr-Tyr-OH (L-Tyrosyl-L-tyrosine) is an antihypertensive peptide. H-Tyr-Tyr-OH inhibits angiotensin I-converting enzyme (ACE) with an IC50 value of 0.028 mg/mL. H-Tyr-Tyr-OH can be used for the research of high blood pressure[1][2].

IC50 & Target

IC50: 0.028 mg/mL (ACE)[2]
In Vitro

H-Tyr-Tyr-OH shows inhibitory effect against ACE with an IC50 value of 0.028 mg/mL[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H-Tyr-Tyr-OH Related Antibodies
In Vivo

H-Tyr-Tyr-OH (12.5 mg/kg; intraarterial injection once) effects systolic blood pressure in vivo[1].
H-Tyr-Tyr-OH (0-100 mg/kg; i.p. once) effects blood pressure of spontaneously hypertensive rats[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Anesthetized male SpragueDawley rats[1]
Dosage: 12.5 mg/kg
Administration: Intraarterial injection; 12.5 mg/kg once
Result: Significantly elevated systolic blood pressure, with a peak increase of 5 min after administration.
Animal Model: Spontaneously hypertensive rats[1]
Dosage: 0, 50 and 100 mg/kg
Administration: Intraperitoneal injection; once
Result: Significantly reduced blood pressure of spontaneously hypertensive rats, even at a dose of 50 mg/kg.
Molecular Weight

344.36

Formula

C18H20N2O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(C[C@H](NC([C@@H](N)CC2=CC=C(C=C2)O)=O)C(O)=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (290.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (290.39 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9039 mL 14.5197 mL 29.0394 mL
5 mM 0.5808 mL 2.9039 mL 5.8079 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥97.0%

COA (246 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.9039 mL 14.5197 mL 29.0394 mL 72.5984 mL
5 mM 0.5808 mL 2.9039 mL 5.8079 mL 14.5197 mL
10 mM 0.2904 mL 1.4520 mL 2.9039 mL 7.2598 mL
15 mM 0.1936 mL 0.9680 mL 1.9360 mL 4.8399 mL
20 mM 0.1452 mL 0.7260 mL 1.4520 mL 3.6299 mL
25 mM 0.1162 mL 0.5808 mL 1.1616 mL 2.9039 mL
30 mM 0.0968 mL 0.4840 mL 0.9680 mL 2.4199 mL
40 mM 0.0726 mL 0.3630 mL 0.7260 mL 1.8150 mL
50 mM 0.0581 mL 0.2904 mL 0.5808 mL 1.4520 mL
60 mM 0.0484 mL 0.2420 mL 0.4840 mL 1.2100 mL
80 mM 0.0363 mL 0.1815 mL 0.3630 mL 0.9075 mL
100 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7260 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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H-Tyr-Tyr-OH Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H-Tyr-Tyr-OH1050-28-8L-Tyrosyl-L-tyrosineAngiotensin-converting Enzyme (ACE)antihypertensive peptidehigh blood pressuresystolic blood pressurespontaneously hypertensive ratInhibitorinhibitorinhibit

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