hCYP3A4 Fluorogenic substrate 1

Name: hCYP3A4 Fluorogenic substrate 1
Brand: MedChemExpress
SKU: HY-155002
Rating: 4.5 (1 reviews)

Cat. No.: HY-155002 Pureza: 99.07%: Ficha de datos
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hCYP3A4 Fluorogenic substrate 1 is a potrnt hCYP3A4 fluorogenic substrate with an K_m value of 0.36 μM. hCYP3A4 Fluorogenic substrate 1 can be used for cell and in vivo imaging.

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hCYP3A4 Fluorogenic substrate 1 Estructura química

No. CAS : 186299-00-3

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 1 publication(s) in Google Scholar

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Cytochrome P450:

Ver todas las isoformas

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

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Revisión del cliente

Descripciòn

hCYP3A4 Fluorogenic substrate 1 is a potrnt hCYP3A4 fluorogenic substrate with an K_m value of 0.36 μM. hCYP3A4 Fluorogenic substrate 1 can be used for cell and in vivo imaging^[1].

IC₅₀ & Target^[1]

CYP3A4

0.36 μM (Km)

In Vitro

hCYP3A4 Fluorogenic substrate 1 (compound F8) (20 μM; 30 min) shows brightly fluorescent signals at the green channel in both living Hep3B2 cells and Huh-7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

hCYP3A4 Fluorogenic substrate 1 (10 mg/kg; i.v.) can be used for imaging in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats^[1]
Dosage:	10 mg/kg
Administration:	I.v.
Result:	Can be used as a probe substrate offers a highly efficient and easy-to-use approach for screening and characterizing hCYP3A4 inhibitors or time-dependent inactivators, which strongly facilitates hCYP3A4-mediated DDI studies.

Peso molecular

305.30

Fòrmula

C₁₉H₁₂FNO₂

No. CAS

186299-00-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C2C(C3=O)=CC=CC2=CC=C1)N3CC4=CC=C(F)C=C4

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 12.5 mg/mL (40.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2755 mL	16.3773 mL	32.7547 mL
5 mM	0.6551 mL	3.2755 mL	6.5509 mL
10 mM	0.3275 mL	1.6377 mL	3.2755 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.07%

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Ficha de datos (276 KB) SDS (393 KB)

COA (255 KB) HNMR (261 KB) RP-HPLC (259 KB) MS (71 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. He RJ, et al. Rationally Engineered CYP3A4 Fluorogenic Substrates for Functional Imaging Analysis and Drug-Drug Interaction Studies. J Med Chem. 2023 May 25;66(10):6743-6755. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2755 mL	16.3773 mL	32.7547 mL	81.8867 mL
	5 mM	0.6551 mL	3.2755 mL	6.5509 mL	16.3773 mL
	10 mM	0.3275 mL	1.6377 mL	3.2755 mL	8.1887 mL
	15 mM	0.2184 mL	1.0918 mL	2.1836 mL	5.4591 mL
	20 mM	0.1638 mL	0.8189 mL	1.6377 mL	4.0943 mL
	25 mM	0.1310 mL	0.6551 mL	1.3102 mL	3.2755 mL
	30 mM	0.1092 mL	0.5459 mL	1.0918 mL	2.7296 mL
	40 mM	0.0819 mL	0.4094 mL	0.8189 mL	2.0472 mL