Nelipepimut-S
Nelipepimut-S (E75) is a peptide vaccine targeting HER2. When used in combination with granulocyte-macrophage colony-stimulating factor, Nelipepimut-S stimulates immune activity and induces anti-HER2 immune responses. Nelipepimut-S can be used in research related to breast cancer, triple-negative breast cancer and ductal carcinoma in situ.
For research use only. We do not sell to patients.
- CAS No.: 160212-35-1
- Formula: C50H78N10O11
- Molecular Weight:995.21
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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HER2 |
Nelipepimut-S acts as the HLA-A2 immunodominant epitope of HER2, potently stimulating cell lysis by ovarian cancer reactive CTL lines and demonstrating high HLA-A2 stabilizing ability[2].
Nelipepimut-S is presented on HLA-A2 in ovarian cancer cell lines and is recognized by ovarian cancer-associated CD8+ T-cell clones[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 160212-35-1
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Molecular Weight 995.21
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Formula C50H78N10O11
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Synonyms
E75
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Sequence Shortening
KIFGSLAFL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
[1]. Dillon PM, et al. Evaluating Nelipepimut-S in the Treatment of Breast Cancer: A Short Report on the Emerging Data. Breast cancer (Dove Medical Press). 2020;12:69-75. [Content Brief]
[2].
Clifton GT, et al. The development and use of the E75 (HER2 369-377) peptide vaccine. Future Oncol. 2016 Jun;12(11):1321-9.
[Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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