1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Cell Apoptosis and Necrosis
  5. Cell Senescence β-Galactosidase Staining Kit

Cell Senescence β-Galactosidase Staining Kit 

Cat. No.: HY-K1089
Manual SDS

MCE Cell Senescence β-Galactosidase Staining Kit can be used to evaluate the senescence of cells or tissues.

Cell Senescence β-Galactosidase Staining Kit
Size Price Stock Quantity
100 T USD 176 In-stock

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

Due to limited replicative lifespan, normal cells enter cell cycle arrest, also known as cellular senescence1. While in this senescence phase the cells remain metabolically active without undergoing cell death or division. These senescent cells adopt a specific phenotypic state that includes the appearance of multinucleated cells, increased vacuolization, expression of pH-dependent β-galactosidase, and morphological changes where cells become enlarged and extended2, 3. Senescence, through a variety of mechanisms, can also play a role in tumor suppression, tumor progression, aging, and tissue repair.

β-galactosidase (also known as β-gal) is an essential hydrolase enzyme that catalyzes the hydrolysis of galactose-containing carbohydrates into monosaccharides. Substrates of β-galactosides include lactose, various glycoproteins, ganglioside GM1, and lactosylceramides. β-galactosidase is used widely in molecular biology; for example, isolation of recombinant bacteria during molecular cloning utilizes α-complementation of the bacterial β-galactosidase gene (lacZ) in the presence of a β-gal substrate to identify recombinant clones. In cell biology, Senescence-Associated beta-galactosidase (SA-β-gal), defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence.

MCE Cell Senescence β-Galactosidase Staining Kit is based on SA-β-gal catalyzing the transformation of X Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, X-gal) into a blue product at pH 6. This allows for the visualization of cell or tissue senescence under the microscope.

Storage

-20℃, 1 year

X Gal solution needs to be stored away from light.

Components
Components HY-K1089-100 T
β-Galactosidase Staining Fixative 100 mL
β-Galactosidase Staining Solution A 1.5 mL
β-Galactosidase Staining Solution B 1.5 mL
β-Galactosidase Staining Solution C 100 mL
X Gal Solution 5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cell Senescence β-Galactosidase Staining Kit
Cat. No.:
HY-K1089
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