Hit20
Hit20 is a PI3Kα selective inhibitor, inhibits PI3Kα kinase activity, suppresses PI3Kα phosphorylation. Hit20 suppresses proliferation, colony formation, migration, and invasion of colon cancer cells. Hit20 can be used for the research of colon cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 536714-52-0
- 分子式: C22H17ClN6O2S2
- 分子量:496.99
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
製品説明
体外実験
Hit20 (10 μM) inhibits PI3Kα kinase activity in a cell-free assay with 40.24% inhibition at 10 μM[1].
Hit20 (0-100 μM; 48 h) inhibits proliferation of HCT-116, MCF-7, A549, and H1299 cells with the highest potency against HCT-116 cells (IC50 7.26 μM)[1].
Hit20 (1, 3, 6 μM; 48 h treatment, 14 days culture) reduces colony formation of HCT-116 cells in a concentration-dependent manner[1].
Hit20 (1, 3, 6 μM; 48 h) inhibits wound healing of HCT-116 cells in concentration- and time-dependent manners[1].
Hit20 (1, 3, 6 μM; 48 h) inhibits migration and invasion of HCT-116 cells in a concentration-dependent manner[1].
Hit20 (3, 6, 9 μM; 48 h) induces apoptosis of HCT-116 cells in a concentration-dependent manner[1].
Hit20 (3, 6, 9 μM; 48 h) modulates expression of apoptosis-related proteins (Caspase-3/9, cleaved Caspase-3/9, Bcl-2, Bax) in HCT-116 cells in a concentration-dependent manner[1].
Hit20 (3, 6, 9 μM; 48 h) inhibits phosphorylation of PI3Kα and Akt in HCT-116 cells in a concentration-dependent manner, suppressing the PI3K/Akt signaling pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:MCF-7, HCT-116, A549, H1299 cells
-
Concentration:7.26 μM (IC50, HCT-116); 27.37 μM (IC50, MCF-7); 13.40 μM (IC50, A549); 12.22 μM (IC50, H1299)
-
Incubation Time:48 h
-
Result:Exhibited the highest anti-proliferative activity against HCT-116 cells with an IC50 of 7.26 μM, while showing weaker activity against MCF-7 (IC50 27.37 μM), A549 (IC50 13.40 μM), and H1299 (IC50 12.22 μM) cells.
-
Cell Line:HCT-116 cells
-
Concentration:1, 3, 6 μM
-
Incubation Time:48 h (treatment); 14 days (culture)
-
Result:Reduced colony formation in a concentration-dependent manner; showed lower colony numbers in Hit20-treated groups compared to the control.
-
Cell Line:HCT-116 cells
-
Concentration:1, 3, 6 μM
-
Incubation Time:48 h
-
Result:Decreased wound healing rates in a concentration- and time-dependent manner; reached 33.66% (1 μM), 26.61% (3 μM), and 24.90% (6 μM) at 48 h compared to the control.
-
Cell Line:HCT-116 cells
-
Concentration:3, 6, 9 μM
-
Incubation Time:48 h
-
Result:Increased total apoptosis rates in a concentration-dependent manner; reached 7.14% (3 μM), 10.51% (6 μM), and 17.89% (9 μM) compared to the control’s 5.86%.
-
Cell Line:HCT-116 cells
-
Concentration:3, 6, 9 μM
-
Incubation Time:48 h
-
Result:Downregulated expression of Caspase-3/9 and Bcl-2, while upregulated cleaved Caspase-3/9 and Bax in a concentration-dependent manner.\nReduced phosphorylation levels of PI3Kα (p85α) and Akt (Ser473) in a concentration-dependent manner, decreased p-PI3K/PI3K and p-Akt/Akt ratios.
化学情報
-
CAS 番号 536714-52-0
-
分子量 496.99
-
分子式 C22H17ClN6O2S2
-
SMILES
CCC1=NN=C(S1)NC(CSC2=NC3=C(C(N2C4=CC=C(C=C4)Cl)=O)NC5=CC=CC=C53)=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Hit20 関連分類
HY-181150 関連分類
Powered by Bioz