Pexiganan TFA
Pexiganan TFA is an antimicrobial peptide with broad-spectrum bactericidal. Pexiganan TFA disrupts bacterial cell membranes, induces peptidoglycan damage and cell lysis. Pexiganan TFA can be used for the research of bacterial infection.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C122H210N32O22·xC2H4O2
- 分子量:2477.17 (free base)
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
Pexiganan (1-256 µg/mL; 24 h) TFA exhibits comparable bactericidal activity against planktonic Staphylococcus aureus Z25.2, Pseudomonas aeruginosa Z25.1, and their dual-species cultures[1].
Pexiganan (1-256 µg/mL; 24 h) TFA is more effective at inhibiting and eradicating Staphylococcus aureus Z25.2 biofilms than Pseudomonas aeruginosa Z25.1 or dual-species biofilms[1].
Pexiganan (7-35 μM) TFA binds to purified bovine type I collagen with a dissociation constant of 34.01 μM[3].
Pexiganan (5-80 μg/mL; overnight) TFA inhibits the growth of Pseudomonas aeruginosa and Staphylococcus aureus[3].
Pexiganan (40 μg/mL; 0-60 min) TFA rapidly reduces viable counts of Pseudomonas aeruginosa (ATCC27853) and Staphylococcus aureus (ATCC29213) over 60 min, with complete elimination of P. aeruginosa by 60 min[3].
Pexiganan (10-50 μg/mL) TFA exhibits no cytotoxicity toward rat fibroblast cells in in vitro MTT assays[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
体内実験
Pexiganan (1 mg/kg; i.p.; single dose) TFA reduces lethality, and intra-abdominal bacterial counts in E. coli-induced septic shock in male Wistar rats[4].
Pexiganan (1 mg/kg; i.p.; single dose) TFA reduces lethality, and intra-abdominal bacterial counts in in cecal ligation and puncture-induced septic shock in male Wistar rats[4].
Pexiganan (1 mg/kg; i.p.; single dose) TFA is well-tolerated with no detectable adverse effects or physiological changes in healthy male Wistar rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats (adult male, 200-300 g, E. coli O111:B4 LPS (HY-D1056A1)-induced septic shock)[4]
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Dosage:1 mg/kg
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Administration:i.p.; single dose
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Result:Reduced plasma endotoxin levels to ≤0.015 EU/mL.
Reduced plasma TNF-α levels to 0.26 ng/mL.
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Animal Model:Wistar rats (adult male, 200-300 g, E. coli-induced septic shock)[4]
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Dosage:1 mg/kg
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Administration:i.p.; single dose
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Result:Resulted in 32.3% lethality, 5/15 positive blood cultures, and intra-abdominal bacterial counts of 6.0 × 103 CFU/mL.
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Animal Model:Wistar rats (adult male, 200-300 g, cecal ligation and puncture-induced septic shock)[4]
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Dosage:1 mg/kg
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Administration:i.p.; single dose
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Result:When administered immediately after surgery, resulted in 32.3% lethality, 5/15 positive blood cultures, and intra-abdominal bacterial counts of 6.9 × 103 CFU/mL.
When administered immediately after surgery, produced significant reductions in plasma endotoxin and TNF-α levels compared to control and β-lactam-treated groups.
When administered 360 minutes after surgery, resulted in 40.0% lethality, 6/15 positive blood cultures, and intra-abdominal bacterial counts of 6.0 × 104 CFU/mL.
When administered 360 minutes after surgery, produced significant reductions in plasma endotoxin and TNF-α levels compared to control and β-lactam-treated groups.
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Animal Model:Wistar rats (adult male, 200-300 g)[4]
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Dosage:1 mg/kg
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Administration:i.p.; single dose
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Result:Showed no drug-related adverse effects.
Caused no changes in measured physiological parameters including leukocyte count, serum creatinine, rectal temperature, pulse, blood pressure, breathing rate, and oxygenation.
化学情報
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分子量 2477.17 (free base)
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分子式 C122H210N32O22·xC2H4O2
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別名
MSI 78 TFA
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配列
Gly-Ile-Gly-Lys-Phe-Leu-Lys-Lys-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Lys-Ile-Leu-Lys-Lys-NH2
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シーケンスの短縮
GIGKFLKKAKKFGKAFVKILKK-NH2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Protein Extraction
Protein extraction uses physical, chemical or biological methods, such as ultrasonic disruption, salting out, cell lysis, electrophoresis, etc., to destroy the cell membrane structure and to separate the proteins from different components according to their characteristics.
純度とドキュメンテーション
参考文献
[1]. Gomes D, et al. Pexiganan in Combination with Nisin to Control Polymicrobial Diabetic Foot Infections. Antibiotics (Basel). 2020;9(3):128. Published 2020 Mar 20. [Content Brief]
[2]. Ghazvini K, et al. Sustain-release lipid-liquid crystal formulations of pexiganan against Helicobacter pylori infection: in vitro evaluation in C57BL/6 mice. BMC Pharmacol Toxicol. 2024;25(1):9. Published 2024 Jan 11. [Content Brief]
[3]. Gopinath D, et al. Pexiganan-incorporated collagen matrices for infected wound-healing processes in rat. J Biomed Mater Res A. 2005;73(3):320-331. [Content Brief]
[4]. Giacometti A, et al. Effects of pexiganan alone and combined with betalactams in experimental endotoxic shock. Peptides. 2005;26(2):207-216. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Pexiganan TFA 関連分類
HY-105088C 関連分類
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