SMO-IN-2
Based on 1 Customer Validation
SMO-IN-2 is a potent smoothened (SMO) inhibitor with an IC50 value of 123.4 nM for hedgehog (Hh) signaling pathway. SMO-IN-2 has antiproliferative activity against human medulloblastoma cell line Daoy. Anticancer activity.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.12%
- CAS 番号: 1822355-27-0
- 分子式: C25H25F4N5O2
- 分子量:503.49
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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HY-15412
HhAntagSMO Antagonist -
HY-100790
Ciliobrevin AHedgehog Inhibitor -
HY-13307
JK184Hedgehog Inhibitor -
HY-124899
Hh-Ag1.5Hedgehog Agonist -
HY-13282
GANT 58GLI Antagonist -
HY-108439
NeurodazineNeurogenesis Inducer -
HY-107551
Hh Pathway-IN-1Hedgehog Inhibitor -
HY-15398C
3-epi-Vitamin D3Hedgehog Pathway Inhibitor -
HY-100515
RobotnikininShh Inhibitor -
HY-122814
Hedgehog IN-1Hedgehog Inhibitor -
HY-107408
SANT 2Hh-Signaling Pathway Antagonist -
HY-115566
S1PL-IN-1Sphingosine-1-phosphate Lyase Inhibitor
生物活性
製品説明
IC50 & Target
IC50: 123.4 nM (Hh)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2058 | IC50 |
>50 μM
Compound: 1
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Antiproliferation activity against human A2058 cells incubated for 72 hrs by MTT assay
Antiproliferation activity against human A2058 cells incubated for 72 hrs by MTT assay
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[PMID: 32063403] |
| Daoy | IC50 |
0.51 μM
Compound: 1
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Antiproliferative activity against human DaOY cells incubated for 72 hrs by MTT assay
Antiproliferative activity against human DaOY cells incubated for 72 hrs by MTT assay
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[PMID: 32063403] |
体外実験
SMO-IN-2 (compound 1) has antiproliferative activity against human medulloblastoma cell line Daoy with an IC50 of 0.51 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1822355-27-0
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性状 Solid
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分子量 503.49
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分子式 C25H25F4N5O2
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Color White to off-white
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SMILES
O=C(N(C)C1CCN(C(C2=CC=CC=C2NC3=CC=NN3C)=O)CC1)C4=CC=C(F)C=C4C(F)(F)F
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外:
DMSO : 10 mg/mL (19.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.99 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9861 mL | 9.9307 mL | 19.8614 mL | 49.6534 mL |
| 5 mM | 0.3972 mL | 1.9861 mL | 3.9723 mL | 9.9307 mL | |
| 10 mM | 0.1986 mL | 0.9931 mL | 1.9861 mL | 4.9653 mL | |
| 15 mM | 0.1324 mL | 0.6620 mL | 1.3241 mL | 3.3102 mL |
SMO-IN-2 関連分類
HY-150564 関連分類
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