L-Azidonorleucine hydrochloride
Based on 1 Customer Validation
L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants . L-Azidonorleucine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity: 99.70%
- CAS No.: 1454334-76-9
- Formula: C6H13ClN4O2
- Molecular Weight:208.65
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
CHO cells transfected with pMaRSC and incubated with L-Azidonorleucine (Anl; 1.5 mM; 6 h) are fixed, permeabilized, and treated with alkyne-TAMRA. The TAMRA fluorescence exclusively in the mCherry-expressing cells is observed, consistent with cell-selective incorporation of Anl into cellular proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1454334-76-9
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Appearance Solid
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Molecular Weight 208.65
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Formula C6H13ClN4O2
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Color White to off-white
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SMILES
N[C@@H](CCCCN=[N+]=[N-])C(O)=O.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 125 mg/mL (599.09 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (479.27 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (270 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Alborz Mahdavi, et al. Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81. [Content Brief]
[2]. Beatriz Alvarez-Castelao, et al. Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nat Protoc. 2019 Feb;14(2):556-575. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.7927 mL | 23.9636 mL | 47.9272 mL | 119.8179 mL |
| 5 mM | 0.9585 mL | 4.7927 mL | 9.5854 mL | 23.9636 mL | |
| 10 mM | 0.4793 mL | 2.3964 mL | 4.7927 mL | 11.9818 mL | |
| 15 mM | 0.3195 mL | 1.5976 mL | 3.1951 mL | 7.9879 mL | |
| 20 mM | 0.2396 mL | 1.1982 mL | 2.3964 mL | 5.9909 mL | |
| 25 mM | 0.1917 mL | 0.9585 mL | 1.9171 mL | 4.7927 mL | |
| 30 mM | 0.1598 mL | 0.7988 mL | 1.5976 mL | 3.9939 mL | |
| 40 mM | 0.1198 mL | 0.5991 mL | 1.1982 mL | 2.9954 mL | |
| 50 mM | 0.0959 mL | 0.4793 mL | 0.9585 mL | 2.3964 mL | |
| 60 mM | 0.0799 mL | 0.3994 mL | 0.7988 mL | 1.9970 mL | |
| 80 mM | 0.0599 mL | 0.2995 mL | 0.5991 mL | 1.4977 mL | |
| 100 mM | 0.0479 mL | 0.2396 mL | 0.4793 mL | 1.1982 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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