Linoleic acid,suitable for cell culture

Name: Linoleic acid,suitable for cell culture
Brand: MedChemExpress
SKU: HY-N0729D
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0729D Purity: 99.08%: Data Sheet
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Linoleic acid is a critical component of polyunsaturated fatty acids. Linoleic acid reduces GSH-PX and T-SOD activities. Linoleic acid functions as a structural component to maintain a certain level of membrane fluidity of the transdermal water barrier of the epidermis. Linoleic acid can cause acute oxidative damage to red blood cells, ultimately leading to partial acute anemia. Linoleic acid can be used in the research of skin diseases associated with linoleic acid deficiency.

For research use only. We do not sell to patients.

Linoleic acid,suitable for cell culture Chemical Structure

CAS No. : 60-33-3

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Linoleic acid,suitable for cell culture:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vivo

Linoleic acid (0.5 mL, 15-90 mmol/L; i.v.; single tail vein injection) induces dose-dependent acute oxidative red blood cell injury in male Sprague-Dawley rats, with the 60 mmol/L dose causing the most significant reductions in Hb (16.8 g/L) and RBC (0.71×10¹²/L) at 24 h post-injection, along with suppressed antioxidant enzyme activity and increased ferryl Hb formation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley (male, 200±20 g)^[1]
Dosage:	0.5 mL, 15 mmol/L; 0.5 mL, 30 mmol/L; 0.5 mL, 60 mmol/L; 0.5 mL, 90 mmol/L
Administration:	i.v.; single tail vein injection
Result:	Reduced Hb by 13.5±2.0 g/L and RBC by 0.45±0.15×10¹²/L at 24 h post-injection (30 mmol/L dose). Decreased GSH-PX, T-SOD, and H₂O₂ levels significantly compared to controls (P < 0.05) at 24 h and 48 h post-injection (30 mmol/L dose). Induced red blood cell morphological changes (spindle shapes, pleomorphism, shrinkage, deformation, rupture), most distinct at 24 h post-injection (30 mmol/L dose). Reduced Hb by 16.8±1.5 g/L and RBC by 0.71±0.18×10¹²/L at 24 h post-injection (60 mmol/L dose). Decreased GSH-PX, T-SOD, and H₂O₂ levels significantly compared to controls (P < 0.05 at 24 h, P < 0.01 at 48 h) post-injection (60 mmol/L dose). Detected a significant absorbance peak at 425 nm (ferryl Hb Soret peak) at 24 h post-injection, with peak intensity declining at 48 h and 72 h (60 mmol/L dose). Caused 50% of rats to die within 5 minutes of injection, presenting with dyspnea, cyanosis, labial haemoptysis, and convulsions (90 mmol/L dose). Reduced Hb and RBC levels at 24 h post-injection, with changes less severe than 30 mmol/L and 60 mmol/L doses (15 mmol/L dose).

Molecular Weight

280.45

Formula

C₁₈H₃₂O₂

CAS No.

60-33-3

Appearance

Liquid (Density: 0.9022 g/cm³)

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\CCCCCCCC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (713.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5657 mL	17.8285 mL	35.6570 mL
5 mM	0.7131 mL	3.5657 mL	7.1314 mL
10 mM	0.3566 mL	1.7828 mL	3.5657 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.08%

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Data Sheet (267 KB) SDS (252 KB)

COA (238 KB) HNMR (238 KB) GC (348 KB)

Handling Instructions (2659 KB)

References

[1]. Yuan T, et al. Linoleic acid induces red blood cells and hemoglobin damage via oxidative mechanism. Int J Clin Exp Pathol. 2015;8(5):5044-5052. Published 2015 May 1. [Content Brief]

[2]. Whelan J, et al. Linoleic acid. Adv Nutr. 2013 May 1;4(3):311-2.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5657 mL	17.8285 mL	35.6570 mL	89.1424 mL
	5 mM	0.7131 mL	3.5657 mL	7.1314 mL	17.8285 mL
	10 mM	0.3566 mL	1.7828 mL	3.5657 mL	8.9142 mL
	15 mM	0.2377 mL	1.1886 mL	2.3771 mL	5.9428 mL
	20 mM	0.1783 mL	0.8914 mL	1.7828 mL	4.4571 mL
	25 mM	0.1426 mL	0.7131 mL	1.4263 mL	3.5657 mL
	30 mM	0.1189 mL	0.5943 mL	1.1886 mL	2.9714 mL
	40 mM	0.0891 mL	0.4457 mL	0.8914 mL	2.2286 mL
	50 mM	0.0713 mL	0.3566 mL	0.7131 mL	1.7828 mL
	60 mM	0.0594 mL	0.2971 mL	0.5943 mL	1.4857 mL
	80 mM	0.0446 mL	0.2229 mL	0.4457 mL	1.1143 mL
	100 mM	0.0357 mL	0.1783 mL	0.3566 mL	0.8914 mL