Gamma-Linolenic acid
Based on 8 publication(s) in Google Scholar
Gamma-linolenic acid (γ-Linolenic acid) is an orally active unsaturated fatty acid. Gamma-linolenic acid exerts anti-inflammatory effects by inhibiting the NF-κB pathway and the phosphorylation of ERK1/2 and JNK. At the same time, it exerts anticancer effects by inducing apoptosis (Apoptosis) in cancer cells. Additionally, Gamma-linolenic acid also has antioxidant and memory-improving effects. It holds promise for research in the fields of inflammation, neurology, and cancer diseases.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 506-26-3
- Formula: C18H30O2
- Molecular Weight:278.43
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Gamma-Linolenic acid
More- Nat Cell Biol. 2026 Jun;28(6):1204-1218. [Abstract]
- Exp Mol Med. 2026 May;58(5):1642-1656. [Abstract]
- Cell Death Dis. 2025 Nov 24;16(1):857. [Abstract]
- J Anal Test. 2024 Dec.
- Cell Rep. 2025 Nov 25;44(11):116496. [Abstract]
- Cancer Cell Int. 2021 Jun 5;21(1):291. [Abstract]
- Eur J Pharmacol. 2023 Apr 15:945:175618. [Abstract]
- Int J Parasitol Drugs Drug Resist. 2024 Jun 6:25:100551. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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RT-PCR
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
ERK1 |
ERK2 |
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Cell Line
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Type | Value | Description | References |
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| CHO-K1 | EC50 |
4.62 μM
Compound: gamma-linolenic acid
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Agonist activity at human FFAR1 expressed in CHO-K1 cells assessed as calcium flux measured for 100 secs by FLIPR assay
Agonist activity at human FFAR1 expressed in CHO-K1 cells assessed as calcium flux measured for 100 secs by FLIPR assay
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[PMID: 26774923] |
Gamma-linolenic acid (10-100 μM, 24 h) can protect PC12 cells from β-Amyloid (25-35) (HY-P0128)-induced damage by alleviating oxidative stress, restoring cell cycle arrest, and blocking caspase-3 activation in PC12 cells[2]. Gamma-linolenic acid (10-100 μM, 24 h) ameliorates β-Amyloid (25-35) (HY-P0128)-induced neuroinflammation in PC12 cells by inhibiting NF-κB and MAPK signaling pathways[2]. Gamma-linolenic acid (100μM, 2 days) inhibits the proliferation and migration of human and rat GBM cells and induces apoptosis in human and rat GBM cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC12 cells
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Concentration:10, 25, 50, 100 μM
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Incubation Time:24 h
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Result:Dose-dependently repressed proinflammatory cytokine inductions in β-Amyloid (25-35) (HY-P0128) PC12 cells, that is restraining β-Amyloid (25-35)-induced the production of TNF-α, NO and PGE2 by suppressing iNOS and COX-2 expression. Decreased β-Amyloid (25-35)-induced phosphorylation of ERK1/2 and JNK in a dose-dependent manner.
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Cell Line:PC12 cells
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Concentration:10, 25, 50, 100 μM
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Incubation Time:24 h
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Result:Protected PC12 cells against β-Amyloid (25-35) (HY-P0128)-induced cell death.
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Cell Line:PC12 cells
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Concentration:10, 25, 50, 100 μM
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Incubation Time:24 h
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Result:Apparently decreased apoptosis to 28.86%, 22.63 %, 14.34%, and 6.77% at 10, 25, 50 and 100 μM, respectively (50 μM Aβ25–35 treatment increased the cellular apoptotic rate to 38.16%).
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Cell Line:C6 cells (rat glioma) and T98G cells (human glioma)
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Concentration:100 μM
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Incubation Time:2 days
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Result:Significantly increased the proportion of apoptotic cells, which was 44.7% higher compared to the control group.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Indomethacin (HY-14397)-induced gastric ulcer Wistar rats model (250 - 260 g)
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Dosage:100 and 150 mg/kg
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Administration:Oral gavage(p.o.), once daily for 14 days
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Result:Reduced levels of COX1, TNF-1, IL-6 and ICAM and increased PGE2 levels. Normalised antioxidant function by modulating MDA, SOD, GSH and CAT.
Chemical Information
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CAS No. 506-26-3
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Appearance Liquid (Density: 0.92 g/cm3)
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Molecular Weight 278.43
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Formula C18H30O2
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Color Colorless to light yellow
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SMILES
CCCCC/C=C\C/C=C\C/C=C\CCCCC(O)=O
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Synonyms
γ-Linolenic acid
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (8)
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Journal Impact Factor
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Most Recent
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Nat Cell Biol
2026 Jun;28(6):1204-1218. PMID: 42174134 -
Exp Mol Med
Cannabidiol triggers fatty acids β-oxidation mediated by Stat2 to facilitate intestinal stem cells regeneration post radiation. [Abstract]2026 May;58(5):1642-1656. PMID: 42120478 -
Cell Death Dis
Elovl7 sensitizes podocytes to ferroptosis in podocytopathy by elongating polyunsaturated fatty acids. [Abstract]2025 Nov 24;16(1):857. PMID: 41285716
Gamma-Linolenic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Nov 24;16(1):857. [Abstract]
Gamma-Linolenic acid (γ-LA) (80 μM; 24 h) significantly exacerbated the ADR-induced reduction in viability of MPC5 cells.
Gamma-Linolenic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Nov 24;16(1):857. [Abstract]
Gamma-Linolenic acid (γ-LA) (80 μM; 24 h) significantly exacerbated the ADR-induced increase in MDA content and decrease in GSH/GSSG levels of MPC5 cells.
Gamma-Linolenic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Nov 24;16(1):857. [Abstract]
Gamma-Linolenic acid (γ-LA) (80 μM; 24 h) exacerbated changes in the expression of genes involved in ferroptosis (Alox15, Ptgs2, Gpx4, Slc7a11), triacylglycerol synthesis (Dgat1, Dgat2, Plin2) and de novo synthesis (Fasn) of MPC5 cells.
Gamma-Linolenic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Nov 24;16(1):857. [Abstract]
Gamma-Linolenic acid (γ-LA) (80 μM; 24 h) induced increase of MDA in MPC5 cells.
Gamma-Linolenic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Nov 24;16(1):857. [Abstract]
Representative images of oil red staining in Gamma-Linolenic acid (γ-LA) (80 μM; 24 h) stimulated MPC5 cells.
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Cell Rep
A generalized strategy to kill leukemic cells by targeting the regulatory systems governing mitochondrial membrane potential. [Abstract]2025 Nov 25;44(11):116496. PMID: 41166305 -
Cancer Cell Int
Construction of a prognostic model with histone modification-related genes and identification of potential drugs in pancreatic cancer. [Abstract]2021 Jun 5;21(1):291. PMID: 34090418 -
Eur J Pharmacol
Polyunsaturated fatty acids drive neutrophil extracellular trap formation in nonalcoholic steatohepatitis. [Abstract]2023 Apr 15:945:175618. PMID: 36841284 -
Int J Parasitol Drugs Drug Resist
Lower micromolar activity of the antifungal imidazoles on the bacterial-type bifunctional aldehyde/alcohol dehydrogenase (AdhE) in Cryptosporidium parvum and in vitro efficacy against the zoonotic parasite. [Abstract]2024 Jun 6:25:100551. PMID: 38875756
Solvent & Solubility
DMSO : 100 mg/mL (359.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.98 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Sergeant S, et al. Gamma-linolenic acid, Dihommo-gamma linolenic, Eicosanoids and Inflammatory Processes. Eur J Pharmacol. 2016 Aug 15;785:77-86. [Content Brief]
[3]. Andreoli Miyake J, et al. Gamma-Linolenic acid alters migration, proliferation and apoptosis in human and rat glioblastoma cells. Prostaglandins Other Lipid Mediat. 2020 Oct;150:106452. [Content Brief]
[4]. Rahimi K, et al. The protective effects of Gamma-linolenic acid against indomethacin-induced gastric ulcer in rats[J]. British Journal of Nutrition, 2024, 131(11): 1844-1851. [Content Brief]
[5]. Khan SA, et al. Gamma-linolenic acid ameliorated glycation-induced memory impairment in rats. Pharm Biol. 2017 Dec;55(1):1817-1823. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.5916 mL | 17.9578 mL | 35.9157 mL | 89.7892 mL |
| 5 mM | 0.7183 mL | 3.5916 mL | 7.1831 mL | 17.9578 mL | |
| 10 mM | 0.3592 mL | 1.7958 mL | 3.5916 mL | 8.9789 mL | |
| 15 mM | 0.2394 mL | 1.1972 mL | 2.3944 mL | 5.9859 mL | |
| 20 mM | 0.1796 mL | 0.8979 mL | 1.7958 mL | 4.4895 mL | |
| 25 mM | 0.1437 mL | 0.7183 mL | 1.4366 mL | 3.5916 mL | |
| 30 mM | 0.1197 mL | 0.5986 mL | 1.1972 mL | 2.9930 mL | |
| 40 mM | 0.0898 mL | 0.4489 mL | 0.8979 mL | 2.2447 mL | |
| 50 mM | 0.0718 mL | 0.3592 mL | 0.7183 mL | 1.7958 mL | |
| 60 mM | 0.0599 mL | 0.2993 mL | 0.5986 mL | 1.4965 mL | |
| 80 mM | 0.0449 mL | 0.2245 mL | 0.4489 mL | 1.1224 mL | |
| 100 mM | 0.0359 mL | 0.1796 mL | 0.3592 mL | 0.8979 mL |
Gamma-Linolenic acid Related Classifications
HY-N7140 Related Classifications
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