2. Immunology/Inflammation
  3. CD3
  4. M701

M701 is a T-cell engager bispecific humanized antibody targeting epithelial cell adhesion molecule (EpCAM) and cluster of differentiation 3 (CD3). M701 binds to EpCAM on tumor cells and CD3 on T cells, thereby linking the two cell populations to achieve targeted cytotoxicity and T cell-mediated cytotoxicity. M701 is applicable to research related to advanced epithelial solid tumors.

For research use only. We do not sell to patients.

CAS No. : 2933295-42-0

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M701 Related Antibodies

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Description

M701 is a T-cell engager bispecific humanized antibody targeting epithelial cell adhesion molecule (EpCAM) and cluster of differentiation 3 (CD3). M701 binds to EpCAM on tumor cells and CD3 on T cells, thereby linking the two cell populations to achieve targeted cytotoxicity and T cell-mediated cytotoxicity. M701 is applicable to research related to advanced epithelial solid tumors[1].

Recommend Isotype Controls
Species Reactivity

Human
IC50 & Target

CD3 & EpCAM
In Vitro

M701 (serially diluted concentrations; 1 h) binds to HCT116 colorectal carcinoma cells with a dissociation constant of 32 nM[1].
M701 (serially diluted concentrations; 1 h) binds to human T cells with a dissociation constant of 33.7 nM[1].
M701 (0-10 μg/mL; 30 min) dose-dependently bridges EpCAM-positive NCI-N87 gastric carcinoma cells and CD3-positive CIK cells, reaching up to 40% double-positive cells at 10 μg/mL[1].
M701 (serially diluted concentrations; 24 h) potently redirects CIK cell lysis to EpCAM-high HCT116, NCI-N87, and KATO-III tumor cells with EC50 values of 1.1, 1.8, and 8.5 ng/mL respectively, shows minimal activity against EpCAM-low SK-OV-3 and MDA-MB-231 cells, and has no activity against EpCAM-negative U87 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

M701 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: EpCAM-expressing tumor cell lines (HCT116, NCI-N87, KATO-III, SK-OV-3, MDA-MB-231), U87 glioblastoma cells, cytokine-induced killer (CIK) cells
Concentration: Serially diluted concentrations
Incubation Time: 24 h (37°C, 5% CO2 incubator)
Result: Potently redirected CIK cell lysis to EpCAM-high HCT116 cells with an EC50 of 1.1 ng/mL.
Potently redirected CIK cell lysis to EpCAM-high NCI-N87 cells with an EC50 of 1.8 ng/mL.
Potently redirected CIK cell lysis to EpCAM-high KATO-III cells with an EC50 of 8.5 ng/mL.
Showed minimal cytotoxicity against EpCAM-low SK-OV-3 cells with an EC50 of 58.7 ng/mL.
Showed minimal cytotoxicity against EpCAM-low MDA-MB-231 cells with an EC50 of 37.8 ng/mL.
Exhibited no cytotoxicity against EpCAM-negative U87 glioblastoma cells.
In Vivo

M701 (0.1-2.5 mg/kg; i.v.; on days 0, 2, and 4) dose-dependently inhibits subcutaneous HCT116 tumor growth in NOD/SCID mice, with the 2.5 mg/kg and 0.5 mg/kg doses achieving the strongest suppression, resulting in mean day-42 tumor volumes of ≤145 mm3[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD/SCID (female, 7-8 weeks old)[1]
Dosage: 0.1 mg/kg; 0.5 mg/kg; 2.5 mg/kg
Administration: i.v.; on days 0, 2, and 4
Result: Recorded mean tumor volume of 828.17 mm3 at 0.1 mg/kg dose on day 42.
Recorded mean tumor volume of 145.15 mm3 at 0.5 mg/kg dose on day 42.
Recorded mean tumor volume of 112.54 mm3 at 2.5 mg/kg dose on day 42.
Showed statistically significant tumor growth inhibition at 0.5 mg/kg and 2.5 mg/kg doses compared to controls.
Gene ID

916  [NCBI] & 4072  [NCBI]

Accession
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

125.8 kDa

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

N/A
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • half-IGG1/scFv-h-CH2-CH3
Biological Activity
  • Immobilized Human EpCAM Protein, His Tag can bind M701. The EC50 for this effect is 638.8 ng/mL.
  • Loaded M701 on ProA biosensor, can bind CD3E-His&CD3D without tag with an affinity constant of 1.73E-08 M as determined in BLI assay.
Purity & Documentation
References
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M701 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

M7012933295-42-0M 701M-701CD3Cluster of differentiation 3epithelial cell adhesion moleculeT cellsmalignant ascitesovarian cancercolorectal cancerHCT116 colorectal carcinoma cellsNCI-N87 gastric carcinoma cellscluster of differentiation 3tumor cellsgastric cancerInhibitorinhibitorinhibit

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