meso-Dihydroguaiaretic acid
Based on 1 Customer Validation
Dihydroguaiaretic acid, is isolated from the fruits of Saururus chinensis with an anti-cancer activty.
For research use only. We do not sell to patients.
- Purity: 98.49%
- CAS No.: 66322-34-7
- Formula: C20H26O4
- Molecular Weight:330.42
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C8166 | CC50 |
24.9 μM
Compound: 19
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Cytotoxicity against human C8166 cells by MTT assay
Cytotoxicity against human C8166 cells by MTT assay
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[PMID: 19413342] |
| C8166 | CC50 |
49.15 μM
Compound: 24
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Cytotoxicity against human C8166 cells by MTT assay
Cytotoxicity against human C8166 cells by MTT assay
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[PMID: 18278869] |
| C8166 | EC50 |
15.61 μM
Compound: 24
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Antiviral activity against HIV1 in human C8166 cells assessed as prevention of virus-induced cytopathic effect
Antiviral activity against HIV1 in human C8166 cells assessed as prevention of virus-induced cytopathic effect
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[PMID: 18278869] |
| C8166 | EC50 |
4 μM
Compound: 19
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Antiviral activity against HIV1 3B in human C8166 cells assessed as inhibition of virus-induced cytopathogenicity
Antiviral activity against HIV1 3B in human C8166 cells assessed as inhibition of virus-induced cytopathogenicity
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[PMID: 19413342] |
| COS-1 | IC50 |
38.98 μM
Compound: 4
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Inhibitory activity against HIV Tat-regulated Transactivation in COS cells.
Inhibitory activity against HIV Tat-regulated Transactivation in COS cells.
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[PMID: 9685238] |
| HeLa | IC50 |
28 μM
Compound: 4
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by WST-8 reduction assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by WST-8 reduction assay
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[PMID: 27210431] |
| HL-60 | IC50 |
41 μM
Compound: 4
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Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 48 hrs by WST-8 reduction assay
Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 48 hrs by WST-8 reduction assay
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[PMID: 27210431] |
| RAW264.7 | IC50 |
20.3 μM
Compound: 16
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Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 24 hrs in presence of LPS by Griess reagent based assay
Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 24 hrs in presence of LPS by Griess reagent based assay
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[PMID: 31642320] |
| Sf9 | IC50 |
17 μM
Compound: 4
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Cytotoxicity against fall armyworm Sf9 cells assessed as reduction in cell viability after 48 hrs by WST-8 reduction assay
Cytotoxicity against fall armyworm Sf9 cells assessed as reduction in cell viability after 48 hrs by WST-8 reduction assay
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[PMID: 27210431] |
Chemical Information
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CAS No. 66322-34-7
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Appearance Solid
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Molecular Weight 330.42
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Formula C20H26O4
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Color White to off-white
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SMILES
COC1=C(O)C=CC(C[C@@H](C)[C@@H](C)CC2=CC(OC)=C(O)C=C2)=C1
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Synonyms
meso-DGA
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (302.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0265 mL | 15.1323 mL | 30.2645 mL | 75.6613 mL |
| 5 mM | 0.6053 mL | 3.0265 mL | 6.0529 mL | 15.1323 mL | |
| 10 mM | 0.3026 mL | 1.5132 mL | 3.0265 mL | 7.5661 mL | |
| 15 mM | 0.2018 mL | 1.0088 mL | 2.0176 mL | 5.0441 mL | |
| 20 mM | 0.1513 mL | 0.7566 mL | 1.5132 mL | 3.7831 mL | |
| 25 mM | 0.1211 mL | 0.6053 mL | 1.2106 mL | 3.0265 mL | |
| 30 mM | 0.1009 mL | 0.5044 mL | 1.0088 mL | 2.5220 mL | |
| 40 mM | 0.0757 mL | 0.3783 mL | 0.7566 mL | 1.8915 mL | |
| 50 mM | 0.0605 mL | 0.3026 mL | 0.6053 mL | 1.5132 mL | |
| 60 mM | 0.0504 mL | 0.2522 mL | 0.5044 mL | 1.2610 mL | |
| 80 mM | 0.0378 mL | 0.1892 mL | 0.3783 mL | 0.9458 mL | |
| 100 mM | 0.0303 mL | 0.1513 mL | 0.3026 mL | 0.7566 mL |
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