Cat. No.: HY-130778

N,N'-Diacetylchitobiose

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N,N'-Diacetylchitobiose is a dimer of β(1,4) linked N-acetyl-D glucosamine. N,N'-Diacetylchitobiose is the hydrolysate of chitin and can be used as alternative carbon source by E. coli. N,N'-Diacetylchitobiose also reverses myocardial depression.

For research use only. We do not sell to patients.

Purity: 99.59%
CAS No.: 35061-50-8
Formula: C₁₆H₂₈N₂O₁₁
Molecular Weight:424.40

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

N,N'-Diacetylchitobiose Chemical Structure

CAS No.: 35061-50-8

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

N,N'-Diacetylchitobiose (10 mM) promotes the growth of wild-type Escherichia coli expressing the cel operon^[2].
N,N'-Diacetylchitobiose (0.6 mM) is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system in Escherichia coli^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

N,N'-Diacetylchitobiose (Chitobiose form is in the research, 50 mg/kg, over 0.5 h, followed by a constant infusion of 5 mg/kg chitobiose (per hour) for 2 h) reverses myocardial depression and reduces norepinephrine requirements in canine E.coli preparations in dogs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 35061-50-8
Appearance Solid
Molecular Weight 424.40
Formula C₁₆H₂₈N₂O₁₁
Color White to off-white
SMILES

O=C[C@H](NC(C)=O)[C@H]([C@@H]([C@@H](CO)O)O[C@H]1[C@H](NC(C)=O)[C@H]([C@@H]([C@@H](CO)O1)O)O)O
Structure Classification
- Saccharides
Initial Source
- Animals
- Microorganisms
Serratia marcescens 2170
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (294.53 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3563 mL

11.7813 mL

23.5627 mL

5 mM

0.4713 mL

2.3563 mL

4.7125 mL

10 mM

0.2356 mL

1.1781 mL

2.3563 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Fangmeng Chu, et al. An optimized cocktail of chitinolytic enzymes to produce N,N'-diacetylchitobiose and N-acetyl-d-glucosamine from defatted krill by-products. Int J Biol Macromol. 2019 Jul 15;133:1029-1034. [Content Brief]

[2]. Keyhani NO, et al. Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14367-71. [Content Brief]

[3]. Keyhani N O, et al. The Chitin Disaccharide, N, N′-Diacetylchitobiose, Is Catabolized byEscherichia coli and Is Transported/Phosphorylated by the Phosphoenolpyruvate: Glycose Phosphotransferase System. Journal of Biological Chemistry, 2000, 275(42): 33084-33090.

[4]. Mink S N, et al. N, N′-diacetylchitobiose, an inhibitor of lysozyme, reverses myocardial depression and lessens norepinephrine requirements in Escherichia coli sepsis in dogs. Shock, 2008, 29(6): 681-687.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.3563 mL	11.7813 mL	23.5627 mL	58.9067 mL
	5 mM	0.4713 mL	2.3563 mL	4.7125 mL	11.7813 mL
	10 mM	0.2356 mL	1.1781 mL	2.3563 mL	5.8907 mL
	15 mM	0.1571 mL	0.7854 mL	1.5708 mL	3.9271 mL
	20 mM	0.1178 mL	0.5891 mL	1.1781 mL	2.9453 mL
	25 mM	0.0943 mL	0.4713 mL	0.9425 mL	2.3563 mL
	30 mM	0.0785 mL	0.3927 mL	0.7854 mL	1.9636 mL
	40 mM	0.0589 mL	0.2945 mL	0.5891 mL	1.4727 mL
	50 mM	0.0471 mL	0.2356 mL	0.4713 mL	1.1781 mL
	60 mM	0.0393 mL	0.1964 mL	0.3927 mL	0.9818 mL
	80 mM	0.0295 mL	0.1473 mL	0.2945 mL	0.7363 mL
	100 mM	0.0236 mL	0.1178 mL	0.2356 mL	0.5891 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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N,N'-Diacetylchitobiose

Bioactive Compound Library Max

Bioactive Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Covalent Screening Library

Anti-Cardiovascular Disease Compound Library

Microbial Metabolite Library

Glucose Metabolism Compound Library

Animal-Sourced Natural Product Library

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Natural Product Library

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

N,N'-Diacetylchitobiose

Bioactive Compound Library Max

Bioactive Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Covalent Screening Library

Anti-Cardiovascular Disease Compound Library

Microbial Metabolite Library

Glucose Metabolism Compound Library

Animal-Sourced Natural Product Library

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Natural Product Library

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

N,N'-Diacetylchitobiose Related Classifications

HY-130778 Related Classifications