Referencia número: HY-W008829

N,N-Diethylacetamide

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N,N-Diethylacetamide is a polar solvent widely used in film and fiber manufacturing, as well as in laboratories as a carrier for water-insoluble chemicals. N,N-Diethylacetamide exerts potent anti-inflammatory effects by inhibiting the NF-κB pathway, suppressing the expression of NO and iNOS, and downregulating key inflammatory cytokines such as TNF-α and IL-6, without affecting the MAPK pathway. N,N-Diethylacetamide can be used to study inflammatory preterm birth.

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No. CAS: 685-91-6
Fòrmula: C₆H₁₃NO
Peso molecular:115.17

Almacenamiento:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

N,N-Diethylacetamide Chemical Structure

No. CAS: 685-91-6

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Actividad biológica
- Descripciòn
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Pureza y Documentación
Referencias
Calculators
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

NF-κB

IL-1β

IL-6

IL-8

CYP2B1

CYP2B2

Ver todos los productos específicos de isoformas NF-κB :

Ver todos los productos específicos de isoformas NO Synthase :

Ver todos los productos específicos de isoformas Interleukin Related :

Ver todos los productos específicos de isoformas TNF Receptor :

Ver todos los productos específicos de isoformas Cytochrome P450 :

In Vitro

N,N-Diethylacetamide (10 mM; 26 h) inhibits LPS (HY-D1056)-stimulated NO secretion in RAW 264.7 cells^[1].
N,N-Diethylacetamide (10 mM; 8 h) reduces LPS-induced iNOS expression in RAW 264.7 cells^[1].
N,N-Diethylacetamide (0.1-10 mM; 26 h) inhibits LPS-stimulated IL-6, IL-8, and MCP-1 secretion in HTR-8/SVneo cells^[1].
N,N-Diethylacetamide (10 mM; 26 h) suppresses LPS-stimulated TNF-α, IL-6, IL-1β, GM-CSF, MCP-1, and IL-10 secretion in RAW 264.7 cells^[1].
N,N-Diethylacetamide (0.1-10 mM; 22 h) inhibits LPS-stimulated cytokine and chemokine secretion in human placental explants^[1].
N,N-Diethylacetamide (10 mM; 2.25 h) prevents LPS-induced IkB-α degradation in RAW 264.7 cells^[1].
N,N-Diethylacetamide (10 mM; 26 h) does not alter LPS-stimulated MAPK pathway protein expression in RAW 264.7 cells^[1].
N,N-Diethylacetamide (10 mM; 26 h) inhibits LPS-stimulated NF-κB transcriptional activity in HEK 293 cells overexpressing TLR4, without affecting AP-1 or C/EBP activity^[1].
N,N-Diethylacetamide (0.5-10 mM; 50 min) was catalyzed for deethylation by purified P4502B1 with a K_m of 3.5 mM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HTR-8/SVneo, RAW 264.7, HEK 293
Concentration:

up to 10 mM
Incubation Time:

2 h (pre-treatment); 24 h (LPS treatment)
Result:

Did not significantly affect the viability of HTR-8/SVneo, RAW 264.7, or HEK 293 cells compared to untreated controls.

Western Blot Analysis^[1]

Cell Line:

RAW 264.7
Concentration:

0.1, 1, 10 mM
Incubation Time:

2 h (pre-treatment); 6 h (LPS treatment)
Result:

Significantly inhibited LPS-induced iNOS expression in RAW 264.7 cells at 10 mM (P < 0.001) compared to LPS control.

ELISA Assay^[1]

Cell Line:

HTR-8/SVneo
Concentration:

0.1, 1, 10 mM
Incubation Time:

2 h (pre-treatment); 24 h (LPS treatment)
Result:

Significantly suppressed LPS-stimulated IL-6 secretion at 10 mM (more effectively than BAY 11-7082), caused a 90% reduction in IL-8 secretion at 10 mM (more effective than BAY 11-7082), and significantly inhibited MCP-1 secretion at 1 and 10 mM.

ELISA Assay^[1]

Cell Line:

RAW 264.7
Concentration:

0.1, 1, 10 mM
Incubation Time:

2 h (pre-treatment); 24 h (LPS treatment)
Result:

Significantly reduced TNF-α (P < 0.0001), IL-6, IL-1β, GM-CSF (>90% reduction), MCP-1, and IL-10 (P < 0.0001) secretion from LPS-stimulated RAW 264.7 cells at 10 mM.

Western Blot Analysis^[1]

Cell Line:

RAW 264.7
Concentration:

0.1, 1, 10 mM
Incubation Time:

2 h (pre-treatment); 15 min (LPS treatment)
Result:

Significantly inhibited LPS-induced IkB-α degradation in RAW 264.7 cells at 10 mM (P < 0.01) compared to LPS control.\nDid not affect LPS-stimulated expression of native or phosphorylated JNK1, ERK1/2, or p38 MAPK in RAW 264.7 cells at any concentration.

In Vivo

N,N-Diethylacetamide (375-750 mg/kg; i.p.; two doses) delays LPS-induced preterm birth in a dose-dependent manner, with 750 mg/kg completely preventing delivery for at least 25 h^[1].
N,N-Diethylacetamide (150-500 mg/kg; i.p.; daily; 3 days) administration to rats induces P4502B1/2 and alters hepatic drug-metabolizing enzyme activities in a dose-dependent manner, with higher doses causing hepatotoxicity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57Bl/6 (timed pregnant, weight 27–36 g)^[1]
Dosage:

375 mg/kg; 750 mg/kg
Administration:

i.p.; two doses (T=-15 min and T=10 h)
Result:

Prevented delivery in all 6 mice treated with 750 mg/kg by 25 h post-LPS injection.
Delayed mean delivery time by approximately two hours and prevented delivery in 2/8 mice treated with 375 mg/kg by 25 h post-LPS injection.

Animal Model:

Sprague-Dawley (male, 5-6 weeks old)^[2]
Dosage:

150, 300 mg/kg; 400-500 mg/kg
Administration:

i.p.; daily; 3 days
Result:

Significantly induced the enzymatic activities related to CYP2B1/2 (such as PROD, 16β-testosterone hydroxylation), and enhanced its own metabolism (DEAC deethylase activity).
Increased the expression of CYP2B1/2 protein, while inhibiting the expression of CYP2C11.

Chemical Information

No. CAS 685-91-6
Appearance Liquid (Density: 0.925 g/cm³)
Peso molecular 115.17
Fòrmula C₆H₁₃NO
Color Colorless to light yellow
SMILES

CCN(C(C)=O)CC
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Gorasiya S, et al. N,N-Diethylacetamide and N,N-Dipropylacetamide inhibit the NF-kB pathway in in vitro, ex vivo and in vivo models of inflammation-induced preterm birth. Sci Rep. 2025;15(1):29861. Published 2025 Aug 14. [Content Brief]

[2]. Silvia M, et al. Microsomal metabolism of N,N-diethylacetamide and N,N-dimethylacetamide and their effects on drug-metabolizing enzymes of rat liver. Biochem Pharmacol. 1994;48(4):717-726. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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N,N-Diethylacetamide

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Ver todos los productos específicos de isoformas NF-κB

Ver todos los productos específicos de isoformas NO Synthase

Ver todos los productos específicos de isoformas Interleukin Related

Ver todos los productos específicos de isoformas TNF Receptor

Ver todos los productos específicos de isoformas Cytochrome P450

Actividad biológica

Chemical Information

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Referencias

Calculators

Help & FAQs

N,N-Diethylacetamide Related Classifications

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