2. Cell Cycle/DNA Damage Epigenetics Apoptosis Autophagy
  3. Sirtuin Apoptosis Autophagy Caspase
  4. NCO-141

NCO-141 is a selective SIRT2 inhibitor with an IC50 of 0.5 μM. NCO-141 induces cell apoptosis via caspase activation and mitochondrial superoxide anion production. NCO-141 induces cell autophagy by increasing LC3-II levels and autophagosome accumulation. NCO-141 is applicable to relevant research on leukemia.

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NCO-141

NCO-141 Chemical Structure

CAS No. : 1382354-26-8

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NCO-141 Related Antibodies

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Description

NCO-141 is a selective SIRT2 inhibitor with an IC50 of 0.5 μM. NCO-141 induces cell apoptosis via caspase activation and mitochondrial superoxide anion production. NCO-141 induces cell autophagy by increasing LC3-II levels and autophagosome accumulation. NCO-141 is applicable to relevant research on leukemia[1].

IC50 & Target[1]

SIRT2

0.5 μM (IC50)

In Vitro

NCO-141 (0.1-100 μM; 72 h) inhibits the growth of S1T, MT-2, Jurkat, and HL60 leukemic cell lines in a dose-dependent manner, with GI50 values of 34.9 μM, 26.4 μM, 36.7 μM, and 12.1 μM, respectively[1].
NCO-141 (0.1-100 μM; 72 h) induces dose-dependent apoptosis in S1T, MT-2, Jurkat, and HL60 leukemic cell lines, with 100 μM treatment resulting in 85.3%, 39.0%, 85.9%, and 86.7% specific annexin V-positive cells, respectively[1].
NCO-141 (10-50 μM; 6 h) induces mitochondrial superoxide generation in S1T, MT-2, Jurkat, and HL60 leukemic cell lines[1].
NCO-141 (50-100 μM; 72 h) activates caspase-3, -8, and -9 in S1T, MT-2, Jurkat, and HL60 leukemic cell lines, but induces caspase-independent cell death as pan-caspase inhibition does not prevent cell death or caspase-associated markers[1].
NCO-141 (72 h) increases acetylated histone H4 levels and promotes nuclear p53 degradation in S1T, MT-2, Jurkat, and HL60 leukemic cell lines[1].
NCO-141 (25-100 μM; 24-48 h) induces autophagy in S1T, MT-2, Jurkat, and HL60 leukemic cell lines by increasing LC3-II levels, promoting autophagosome accumulation, and inhibiting autophagosome degradation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NCO-141 Related Antibodies

Cell Viability Assay[1]

Cell Line: HTLV-1-infected CD4+ T-cell line S1T, HTLV-1-infected T-cell line MT-2, T-lineage acute lymphoblastic leukemia cell line Jurkat, acute myeloid leukemia cell line HL60
Concentration: 0.1, 1, 10 and 100 μM
Incubation Time: 72 h
Result: Inhibited the growth of all four leukemic cell lines in a dose-dependent manner.
Achieved GI50 values of 34.9 μM for S1T cells, 26.4 μM for MT-2 cells, 36.7 μM for Jurkat cells, and 12.1 μM for HL60 cells.

Apoptosis Analysis[1]

Cell Line: S1T, MT-2, Jurkat, HL60 cell lines
Concentration: 0.1, 1, 5, 10, 25, 50 and 100 μM
Incubation Time: 72 h
Result: Induced dose-dependent increases in annexin V-positive cells across all four cell lines.
At 100 μM, reached percentages of specific annexin V-positive cells of 85.3% for S1T cells, 39.0% for MT-2 cells, 85.9% for Jurkat cells, and 86.7% for HL60 cells.
Induced early-phase apoptosis (annexin V+/7-amino-actinomycin D- cells) and DNA fragmentation via TUNEL assay.

Cell Autophagy Assay[1]

Cell Line: S1T, MT-2, Jurkat, HL60 cell lines
Concentration: 25, 50 and 100 μM (48 h incubation); 50 μM (24 h incubation)
Incubation Time: 24 h (autophagic flux analysis); 48 h (Cyto-ID analysis, western blot)
Result: Increased autophagy levels (detected via increased Cyto-ID fluorescence) in all four cell lines.
Significantly increased LC3-II (autophagic vesicle-associated) levels compared to untreated controls.
Induced LC3 translocation, and analysis of autophagic flux showed NCO-141 both promotes autophagosome formation and inhibits autophagosome degradation.
Molecular Weight

350.39

Formula

C21H19FN2O2
CAS No.
SMILES

O=C(N)C1=CC=CC=C1NC2=CC=CC(OCCC3=CC=CC(F)=C3)=C2
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Purity & Documentation
References
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NCO-141 Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NCO-1411382354-26-8NCO141NCO 141SirtuinApoptosisAutophagyCaspaseSIRT1HL60SIRT2mitochondrial superoxidecaspaseautophagyHTLV-1-transformed T-cellsapoptosisleukemic cellsadult T-cell leukemia/lymphomaInhibitorinhibitorinhibit

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