Negundoside 

Negundoside is an iridoid glycoside compound. Negundoside exhibits hepatoprotective effects, reduces ROS, lipid peroxidation and intracellular calcium ion levels, and prevents the decrease of mitochondrial membrane potential (MMP) and apoptosis (apoptosis). Negundoside has neuroprotective effects, improves behavioral deficits, alleviates oxidative damage, and ameliorates cerebral infarction. Negundoside also possesses antibacterial and antiparasitic activities^[1][2][3][4].

Negundoside (5-400 mg/L; 1-24 h) shows no toxicity to HuH-7 cells and antagonizes CCl₄-induced cell death, with a PC₅₀ of 24.46 mg/L^[2].
Negundoside (30-100 mg/L; 25 h) protects HuH-7 cells from CCl₄-induced morphological damage to cells and nuclei, including apoptosis-like changes^[2].
Negundoside (5-100 mg/L; 1 h) dose-dependently stabilizes human erythrocyte membranes against Triton X-100-induced hemolysis, with a protective rate of 91% at 100 mg/L^[2].
Negundoside (5-100 mg/L; 20 min-24 h) dose-dependently inhibits lipid peroxidation (LPO) in rat liver microsomes treated with FeSO₄/H₂O₂ and HuH-7 cells treated with CCl₄, with maximum inhibition rates of 69% and 131% at the concentration of 100 mg/L, respectively^[2].
Negundoside (10-100 mg/L; 25 h) dose-dependently inhibits CCl₄-induced ROS and H₂O₂ production in HuH-7 cells, with a maximum inhibition rate of 104% for ROS and 143% for H₂O₂ at 100 mg/L^[2].
Negundoside (10-100 mg/L; 25 h) dose-dependently reduces CCl₄-induced intracellular Ca²⁺ elevation in HuH-7 cells, with an inhibition rate of up to 106% at 100 mg/L^[2].
Negundoside (10-100 mg/L; 25 h) inhibits the activation of caspase-3 in HuH-7 cells in a dose-dependent manner, with an inhibition rate of 139% at the concentration of 100 mg/L^[2].
Negundoside (50-100 mg/L; 40 min) inhibits cytochrome C release in isolated rat liver mitochondria, with an inhibition rate of up to 105% at 100 mg/L^[2].
Negundoside (50-100 mg/L; 25 h) protects HuH-7 cells against CCl₄-induced loss of mitochondrial membrane potential, reducing the proportion of damaged mitochondria to 20% and 11%, respectively^[2].
Negundoside (25-100 mg/L; 25 h) inhibits CCl₄-induced apoptosis in HuH-7 cells, as evidenced by reduced DNA fragmentation and a decreased proportion of cells in the sub-G₀/G₁ phase (down to 11% at 100 mg/L)^[2].
Negundoside (10-100 mg/L; 25 h) restores the reduced cAMP level in HuH-7 cells treated with CCl₄^[2].
Negundoside (10-100 mg/L; 25 h) dose-dependently inhibits CCl₄-induced activation of cPLA₂ in HuH-7 cells, with an inhibition rate reaching 304% at 50 mg/L^[2].
Negundoside (5-100 mg/L; 25 h) dose-dependently restores the depleted intracellular GSH levels in HuH-7 cells treated with CCl₄, with a recovery rate of up to 147% at 100 mg/L^[2].
Negundoside (12.5 μg/mL) inhibits the growth of Bacillus subtilis with an MIC of 12.5 μg/mL; it produces an 18 mm inhibition zone against Staphylococcus aureus, Micrococcus pyogenes, Pseudomonas aeruginosa and Escherichia coli in vitro^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Negundoside (1-15 mg/kg; i.p.; single administration) exerts dose-dependent neuroprotective efficacy against global cerebral ischemia-reperfusion injury in Balb/C mice^[4]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

496.46

C₂₃H₂₈O₁₂

82451-20-5

OC(C1=CO[C@@H](O[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CO)O)O)OC(C3=CC=C(C=C3)O)=O)[C@@]4([H])[C@]1([H])CC[C@@]4(O)C)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kabilan SJ, et al. Pharmacoinformatics-based screening of active compounds from Vitex negundo against lymphatic filariasis by targeting asparaginyl-tRNA synthetase. J Mol Model. 2023;29(4):87. Published 2023 Mar 6.  [Content Brief]

  [2]. Tasduq SA, et al. Negundoside, an irridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride. World J Gastroenterol. 2008;14(23):3693-3709.  [Content Brief]

  [3]. Islam Z, et al. Vitex Genus as a Source of Antimicrobial Agents. Plants (Basel). 2024 Jan 29;13(3):401.  [Content Brief]

  [4]. Mehta K, et al. Vitex negundo protects against cerebral ischemia-reperfusion injury in mouse via attenuating behavioral deficits and oxidative damage. Psychopharmacology (Berl). 2022;239(2):573-587.  [Content Brief]