1. Cell Cycle/DNA Damage
    PI3K/Akt/mTOR
  2. CDK
    ATM/ATR
  3. NU6027

NU6027 

Cat. No.: HY-13816
Handling Instructions

NU6027 is a potent and ATP-competitive inhibitor of both CDK1 and CDK2, with Kis of 2.5 µM and 1.3 µM, respectively. NU6027 is also a potent inhibitor of ATR and enhances hydroxyurea and cisplatin cytotoxicity in an ATR-dependent manner.

For research use only. We do not sell to patients.

NU6027 Chemical Structure

NU6027 Chemical Structure

CAS No. : 220036-08-8

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Description

NU6027 is a potent and ATP-competitive inhibitor of both CDK1 and CDK2, with Kis of 2.5 µM and 1.3 µM, respectively. NU6027 is also a potent inhibitor of ATR and enhances hydroxyurea and cisplatin cytotoxicity in an ATR-dependent manner[1][2].

IC50 & Target[1][2]

CDK1

2.5 μM (Ki)

CDK2

1.3 μM (Ki)

ATR

 

In Vitro

NU6027 (1 nM-100 µM; 48 h) inhibits the growth of human tumor cells with a GI50 of 10±6 µM[1].
NU6027 (0.1-25 µM; 24 h) inhibits ATR activity with an IC50 of 2.8 µM in GM847KD cells. NU6027 (1-10 µM; 24 h) inhibits ATR activity with an IC50 of 6.7±2.3 µM in MCF7 cells[2].
NU6027 (4 or 10 µM; 24 h) attenuates G2/M arrest following DNA damage in MCF7 cells[2].
NU6027 (10 µM; 24 h) significantly reduces RAD51 foci in both control and PF-01367338-treated V-C8 B2 cells[2].
NU6027 (4 µM; 24 h) causes 82% suppression of the increase in RAD51 foci-positive cells treated by PF-01367338[2].

Western Blot Analysis[2]

Cell Line: MCF7 cells
Concentration: 0, 1, 5, 10 μM
Incubation Time: 24 h
Result: Inhibited CDK2-mediated pRbT821 by 42±27% compared with 70±12% inhibition of pCHK1S345 with the concentration of 10 µM.
Molecular Weight

251.28

Formula

C₁₁H₁₇N₅O₂

CAS No.

220036-08-8

SMILES

NC1=NC(OCC2CCCCC2)=C(N=O)C(N)=N1

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

NU6027NU 6027NU-6027CDKATM/ATRCyclin dependent kinaseAtaxia telangiectasia mutatedATM and RAD3 relatedCDK1CDK2ATRhydroxyureacisplatincytotoxicitycancerInhibitorinhibitorinhibit

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