O,O-Dimethyl-cannabigerol
Based on 1 Customer Validation
O,O-Dimethyl-cannabigerol is a natural product from Cannabis sativa. O,O-Dimethyl-cannabigerol has an antibacterial effect on drug-resistant strains of Staphylococcus aureus (MIC ranging from 1 to 2 μg/mL). O,O-Dimethyl-cannabigerol is a nonpsychoactive constituent.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 29106-16-9
- Formula: C23H36O2
- Molecular Weight:344.53
-
Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
O,O-Dimethyl-cannabigerol (compound 1) inhibits KB cells growth with IC50s of 44.55 μM (MTT assay) and 69.4 μM (SRB assay)[2].
O,O-Dimethyl-cannabigerol (0-100 μM; 48 hours) induces cell cytotoxic in NIH 3T3 fibroblasts, with CD50s of 60.46 μM (MTT assay) and 82.98 μM (SRB assay)[2].
O,O-Dimethyl-cannabigerol shows antitumor efficacy against melanoma cells of a mouse's skin with an IC50 of 31.3 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:NIH 3T3 fibroblasts
-
Concentration:0, 1, 25, 50, 100 μM
-
Incubation Time:48 hours
-
Result:Induced cell cytotoxic with CD50s of 60.46 μM (MTT assay) and 82.98 μM (SRB assay)
Chemical Information
-
CAS No. 29106-16-9
-
Appearance Liquid (Density: 0.927±0.06 g/cm3)
-
Molecular Weight 344.53
-
Formula C23H36O2
-
Color Light yellow to yellow
-
SMILES
CCCCCC1=CC(OC)=C(C/C=C(C)/CC/C=C(C)\C)C(OC)=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 55 mg/mL (159.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.75 mg/mL (7.98 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.75 mg/mL (7.98 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (270 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Appendino G, et al. Antibacterial cannabinoids from Cannabis sativa: a structure-activity study. J Nat Prod. 2008;71(8):1427-1430. [Content Brief]
[2]. Han DS, et al. Synthesis and cytotoxic effects of deoxy-tomentellin. Arch Pharm Res. 2000;23(2):121-127. [Content Brief]
[3]. Caprioglio D, et al. O-Methyl Phytocannabinoids: Semi-synthesis, Analysis in Cannabis Flowerheads, and Biological Activity. Planta Med. 2019;85(11-12):981-986. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9025 mL | 14.5125 mL | 29.0250 mL | 72.5626 mL |
| 5 mM | 0.5805 mL | 2.9025 mL | 5.8050 mL | 14.5125 mL | |
| 10 mM | 0.2903 mL | 1.4513 mL | 2.9025 mL | 7.2563 mL | |
| 15 mM | 0.1935 mL | 0.9675 mL | 1.9350 mL | 4.8375 mL | |
| 20 mM | 0.1451 mL | 0.7256 mL | 1.4513 mL | 3.6281 mL | |
| 25 mM | 0.1161 mL | 0.5805 mL | 1.1610 mL | 2.9025 mL | |
| 30 mM | 0.0968 mL | 0.4838 mL | 0.9675 mL | 2.4188 mL | |
| 40 mM | 0.0726 mL | 0.3628 mL | 0.7256 mL | 1.8141 mL | |
| 50 mM | 0.0581 mL | 0.2903 mL | 0.5805 mL | 1.4513 mL | |
| 60 mM | 0.0484 mL | 0.2419 mL | 0.4838 mL | 1.2094 mL | |
| 80 mM | 0.0363 mL | 0.1814 mL | 0.3628 mL | 0.9070 mL | |
| 100 mM | 0.0290 mL | 0.1451 mL | 0.2903 mL | 0.7256 mL |
O,O-Dimethyl-cannabigerol Related Classifications
HY-137960 Related Classifications
Powered by Bioz