Prosapogenin A
Based on 1 Customer Validation
Prosapogenin A, a natural product from Veratrum, induces apoptosis in human cancer cells in vitro via inhibition of the STAT3 signaling pathway and glycolysis.
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- Pureza : 99.88%
- No. CAS: 19057-67-1
- Fòrmula: C39H62O12
- Peso molecular:722.90
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BXPC-3 | GI50 |
0.43 μg/mL
Compound: 5
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Growth inhibition of humanBxPC3 cells after 48 hrs
Growth inhibition of humanBxPC3 cells after 48 hrs
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[PMID: 15921418] |
| DU-145 | GI50 |
>10 μg/mL
Compound: 5
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Growth inhibition of human DU145 cells after 48 hrs
Growth inhibition of human DU145 cells after 48 hrs
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[PMID: 15921418] |
| HeLa | IC50 |
6.23 μg/mL
Compound: 7
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Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
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[PMID: 15620239] |
| HL-60 | IC50 |
5 μM
Compound: 25
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Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
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[PMID: 18023191] |
| KM-20L2 | GI50 |
1.2 μg/mL
Compound: 5
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Growth inhibition of human KM20L2 cells after 48 hrs
Growth inhibition of human KM20L2 cells after 48 hrs
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[PMID: 15921418] |
| MCF7 | GI50 |
0.26 μg/mL
Compound: 5
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Growth inhibition of human MCF7 cells after 48 hrs
Growth inhibition of human MCF7 cells after 48 hrs
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[PMID: 15921418] |
| NCI-H460 | GI50 |
1.8 μg/mL
Compound: 5
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Growth inhibition of human NCI-H460 cells after 48 hrs
Growth inhibition of human NCI-H460 cells after 48 hrs
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[PMID: 15921418] |
| NCI-H460 | IC50 |
5.04 μM
Compound: 15
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Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
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[PMID: 28606759] |
| P388 | ED50 |
>10 μg/mL
Compound: 5
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Growth inhibition of mouse P388 cells after 48 hrs
Growth inhibition of mouse P388 cells after 48 hrs
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[PMID: 15921418] |
| SF-268 | GI50 |
1.6 μg/mL
Compound: 5
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Growth inhibition of human SF268 cells after 48 hrs
Growth inhibition of human SF268 cells after 48 hrs
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[PMID: 15921418] |
| SK-MEL-1 | IC50 |
7 μM
Compound: 25
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Cytotoxicity against human SKMEL1 cells after 72 hrs by MTT assay
Cytotoxicity against human SKMEL1 cells after 72 hrs by MTT assay
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[PMID: 18023191] |
| U-937 | IC50 |
6 μM
Compound: 25
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Cytotoxicity against human U937 cells after 72 hrs by MTT assay
Cytotoxicity against human U937 cells after 72 hrs by MTT assay
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[PMID: 18023191] |
In Vitro
Prosapogenin A (0-15 µM, 48 h) arrests the cell cycle of HepG2 at G2/M phase, inhibits the growth of cancer cells HeLa, MCF-7 and HepG2 with IC50s of 9.27, 9.36 and 8.41 µM, respectively[1].
Prosapogenin A (2–10 µM, 24 h) activates V-ATPase and caspase 3/8 pathway, triggers increased lysosomal membrane permeability (LMP), and induces a GSDME-dependent pyroptosis in anaplastic thyroid cancer cells 8505C, KHM-5M, C643, BHT-101, and HTh-7[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2
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Concentration:0-15 µM
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Incubation Time:48 h
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Result:Arrested the cell cycle at G2/M phase.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:8505C xenograft mouse models[2]
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Dosage:10-20 mg/kg
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Administration:ip, once daily for 14 days
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Result:Inhibited tumor growth.
Chemical Information
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No. CAS 19057-67-1
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Appearance Solid
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Peso molecular 722.90
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Fòrmula C39H62O12
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Color White to off-white
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SMILES
C[C@@]12[C@]3([H])[C@](O[C@]4(CC[C@@H](C)CO4)[C@H]3C)([H])C[C@@]1([H])[C@@]5([H])[C@]([C@@]6(C(C[C@@H](O[C@@]7([H])[C@@H]([C@H]([C@H](O)[C@@H](CO)O7)O)O[C@@]8([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O8)O)O)CC6)=CC5)C)([H])CC2
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Synonyms
Progenin III
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (138.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.46 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (288 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Wang TX, et al. Prosapogenin A induces apoptosis in human cancer cells in vitro via inhibition of the STAT3 signaling pathway and glycolysis. Oncol Lett. 2013 Nov;6(5):1323-1328. [Content Brief]
[2]. Liu Y, et al., Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification. Cell Death Dis. 2024 Aug 13;15(8):586. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3833 mL | 6.9166 mL | 13.8332 mL | 34.5829 mL |
| 5 mM | 0.2767 mL | 1.3833 mL | 2.7666 mL | 6.9166 mL | |
| 10 mM | 0.1383 mL | 0.6917 mL | 1.3833 mL | 3.4583 mL | |
| 15 mM | 0.0922 mL | 0.4611 mL | 0.9222 mL | 2.3055 mL | |
| 20 mM | 0.0692 mL | 0.3458 mL | 0.6917 mL | 1.7291 mL | |
| 25 mM | 0.0553 mL | 0.2767 mL | 0.5533 mL | 1.3833 mL | |
| 30 mM | 0.0461 mL | 0.2306 mL | 0.4611 mL | 1.1528 mL | |
| 40 mM | 0.0346 mL | 0.1729 mL | 0.3458 mL | 0.8646 mL | |
| 50 mM | 0.0277 mL | 0.1383 mL | 0.2767 mL | 0.6917 mL | |
| 60 mM | 0.0231 mL | 0.1153 mL | 0.2306 mL | 0.5764 mL | |
| 80 mM | 0.0173 mL | 0.0865 mL | 0.1729 mL | 0.4323 mL | |
| 100 mM | 0.0138 mL | 0.0692 mL | 0.1383 mL | 0.3458 mL |
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