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Cat. No.: HY-W1061291

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Quasar670 Phosphoramidite

Quasar670 Phosphoramidite is a modified phosphoramidite monomer that can be used for oligonucleotide synthesis and fluorescent in situ hybridization probe synthesis. Oligonucleotide probes synthesized with Quasar670 Phosphoramidite can be used to detect eGFP mRNA in fixed cells; they can target the 26S rRNA D1/D2 region of Hanseniaspora uvarum to enable tracking and detection of this yeast strain.

For research use only. We do not sell to patients.

CAS No.: 3101697-53-1
Formula: C₄₈H₆₉F₆N₅O₃P₂
Molecular Weight:940.03

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Quasar670 Phosphoramidite Chemical Structure

CAS No.: 3101697-53-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
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- In Vitro
Chemical Information
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Biological Activity

Description

All DNA/RNA Synthesis Isoforms :

In Vitro

Quasar670 Phosphoramidite enables visualization of eGFP mRNA in fixed HEK293T cells; distinguishes HEK293T cells that express eGFP from those that do not in flow-fluorescence in situ hybridization (flow-FISH) experiments^[1].
Quasar670 Phosphoramidite-labelled 9-mer oligonucleotide combinatorial library (MIX-Q) effectively hybridizes to immobilized Cucumis sativus chloroplast DNA probes on epoxy-functionalized glass slides^[2].
The Quasar670 Phosphoramidite-labeled Huv probe enables specific labeling and detection of Hanseniaspora uvarum cells for monitoring population dynamics during wine fermentation via fluorescence microscopy and flow cytometry^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 3101697-53-1
Molecular Weight 940.03
Formula C₄₈H₆₉F₆N₅O₃P₂
SMILES

O=C(CCCCCN1C2=C(C(C)(C)/C1=C\C=C\C=C\C(C(C)(C3=C4C=CC=C3)C)=[N+]4CC)C=CC=C2)NC5CCC(OP(N(C(C)C)C(C)C)OCCC#N)CC5.F[P-](F)(F)(F)(F)F
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Raddaoui N, Croce S, Geiger F, et al. Supersensitive Multifluorophore RNA-FISH for Early Virus Detection and Flow-FISH by Using Click Chemistry. ChemBioChem, 2020, 21(15): 2214-2218.

[2]. Markiewicz W. Research paper Application of epoxy functional silanes in the preparation of DNA microarrays. BioTechnologia, 2014.

[3]. Li L, et al. The impact of simultaneous inoculation with Torulaspora delbrueckii and Hanseniaspora uvarum combined with Saccharomyces cerevisiae on chemical and sensory quality of Sauvignon blanc wines. Frontiers in Microbiology, 2024, 15:1413650.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
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- In Vitro
Chemical Information
Purity & Documentation
References

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Quasar670 Phosphoramidite

All DNA/RNA Synthesis Isoforms