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  5. CD45 Protein, Mouse (HEK293, Fc)

CD45 Protein, Mouse (HEK293, Fc)

Cat. No.: HY-P72916
COA Handling Instructions

The CD45 protein is a multifunctional member of the protein tyrosine phosphatase family with heparan sulfate proteoglycan and heparin binding activity as well as protein tyrosine phosphatase activity. It plays a crucial role in lymphocyte differentiation, macromolecular metabolic processes, and signal transduction regulation. CD45 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD45 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD45 Protein, Mouse (HEK293, Fc) is 400 a.a., with molecular weight of ~130 kDa.

For research use only. We do not sell to patients.

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Description

The CD45 protein is a multifunctional member of the protein tyrosine phosphatase family with heparan sulfate proteoglycan and heparin binding activity as well as protein tyrosine phosphatase activity. It plays a crucial role in lymphocyte differentiation, macromolecular metabolic processes, and signal transduction regulation. CD45 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD45 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD45 Protein, Mouse (HEK293, Fc) is 400 a.a., with molecular weight of ~130 kDa.

Background

CD45 protein, a member of the protein tyrosine phosphatase family, exhibits versatile functions, including heparan sulfate proteoglycan binding activity, heparin binding activity, and protein tyrosine phosphatase activity. It is intricately involved in key processes such as lymphocyte differentiation, positive regulation of macromolecule metabolic processes, and regulation of signal transduction. CD45 acts upstream in lymphocyte-related pathways, including differentiation and activation, while being prominently located in the external side of the plasma membrane, focal adhesion, and membrane raft. Widely expressed in various structures such as the 3rd branchial arch, alimentary system, cardiovascular system, hemolymphoid system, and placenta, CD45's significance is underscored by its association with conditions like systemic lupus erythematosus. The human ortholog of this gene, PTPRC (protein tyrosine phosphatase receptor type C), is implicated in hepatitis C, multiple sclerosis, and severe combined immunodeficiency, highlighting its pivotal role in immune-related disorders.

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

NP_035340.3 (Q26-N425)

Gene ID

19264  [NCBI]

Molecular Construction
N-term
CD45 (Q26-N425)
Accession # NP_035340.3
hFc
C-term
Synonyms
Receptor-type tyrosine-protein phosphatase C; L-CA; T200; PTPRC; CD45
AA Sequence

QTPTPSDGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFN

Molecular Weight

Approximately 130 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD45 Protein, Mouse (HEK293, Fc)
Cat. No.:
HY-P72916
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