Ephrin-A5/EFNA5 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Ephrin-A5/EFNA5 Protein, a cell surface GPI-bound ligand, crucially interacts with Eph receptors, inducing bidirectional signaling and regulating adhesion, organization, and development in neurons, vasculature, and epithelium. It forms complexes with EPHA3, EPHA8, and ADAM10, impacting internalization and function, while also mediating communication in pancreatic islet cells and influencing brain development. Ephrin-A5/EFNA5 Protein, Human (HEK293, His) is the recombinant human-derived Ephrin-A5/EFNA5 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Ephrin-A5/EFNA5 Protein, a cell surface GPI-bound ligand, crucially interacts with Eph receptors, inducing bidirectional signaling and regulating adhesion, organization, and development in neurons, vasculature, and epithelium. It forms complexes with EPHA3, EPHA8, and ADAM10, impacting internalization and function, while also mediating communication in pancreatic islet cells and influencing brain development. Ephrin-A5/EFNA5 Protein, Human (HEK293, His) is the recombinant human-derived Ephrin-A5/EFNA5 protein, expressed by HEK293 , with C-His labeled tag.
Background
Ephrin-A5/EFNA5 Protein is a cell surface GPI-bound ligand that plays a crucial role in neuronal, vascular, and epithelial development by interacting with Eph receptors, a family of receptor tyrosine kinases. This interaction leads to contact-dependent bidirectional signaling between adjacent cells. Ephrin-A5/EFNA5 induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to its cognate receptor, and this signaling requires the activity of the Fyn tyrosine kinase. It activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization, and it may also be involved in maintaining lens transparency and stimulating axon fasciculation. Furthermore, Ephrin-A5/EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion and modulates brain development by regulating cell-cell adhesion and repulsion through its interaction with EPHA7. Additionally, Ephrin-A5/EFNA5 binds to EPHB2 and interacts with EPHA8, activating the latter. It also forms a complex with EPHA2, EPHA3, and ADAM10, which regulates the shedding and internalization of Ephrin-A5/EFNA5, thereby influencing its function.
Verified Bioactivity
Measured by its binding ability in a functional ELISA . Immobilized human EPHA5 at 20 μg/ml (100 μl/well) can bind human EFNA4 with a linear range of 1.28-32 ng/ml.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-His
-
Accession
P52803 (Q21-N203)
-
Molecular Construction
-
N-term
-
EFNA5 (Q21-N203)
Accession # P52803 -
His
-
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
EFNA5; LERK-7; Prev. EPLG7; Alternative Protein EFNA5; EPH-Related Receptor Tyrosine Kinase Ligand 7; GLC1M; LERK7; EFL5; Ephrin-A5; RAGS; AF1; Ephrin A5; AL-1
-
AA Sequence
AQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCEASVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGN
-
Predicted Molecular Mass
23 kDa
-
Molecular Weight
Approximately 27 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (263 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
