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HGF Protein, Human (HEK293)

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Based on 1 Customer Validation

HGF Protein is a hepatocyte growth factor involved in signaling pathways including MET and MAPK.The HGF Protein consists of an alpha chain containing an n-terminal domain four kringle domains, and a β serine protease domain.HGF Protein, Human (HEK293) is the recombinant Virus-derived HGF protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HGF Protein, Human (HEK293)
  • Experimental Validation Results for HGF Protein, Human (HEK293)
  • HGF Protein, Human (HEK293) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

HGF Protein is a hepatocyte growth factor involved in signaling pathways including MET and MAPK.The HGF Protein consists of an alpha chain containing an n-terminal domain four kringle domains, and a β serine protease domain.HGF Protein, Human (HEK293) is the recombinant Virus-derived HGF protein, expressed by HEK293 , with tag free.

Background

Hepatocyte growth factor (HGF) is a potent mitogen of liver cells that acts as a growth factor for a wide range of tissue and cell types. HGF is an activating ligand of Met receptor tyrosine kinase, whose activity is essential for normal cell development and organ regeneration, but abnormal activation of Met is associated with the growth, invasion, and metastasis of many types of solid tumors. HGF activates MAPK signals after TMPRSS13 cutting and activation. HGF is a double-stranded protein consisting of 728 amino acids, the α chain contains an n-terminal domain and four kringle domains, and the β chain contains a serine protease domain. HGF, a member of the plasminogen related growth factor family, is secreted as an inactive single-chain precursor that is proteolytic to form biologically active disulfide-linked α/β heterodimers[1][2][3][4].

Verified Bioactivity

1.Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is <3.0 ng/mL.
2.Measured in a cell proliferation assay using 4MBr-5 cells. The ED50 for this effect is 10.68 ng/mL, corresponding to a specific activity is 9.363×104 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag Tag Free
  • Accession

    P14210-1 (Q32-S728)

  • Gene ID
    3082
  • Molecular Construction
    • N-term
    • HGF (Q32-S728)
      Accession # P14210
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    HGF; Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor); Prev. DFNB39; Lung Fibroblast-Derived Mitogen; HPTA; Hepatopoietin-A; SF; Scatter Factor; F-TCF; Deafness, Autosomal Recessive 39; HGFB; Hepatopoietin A; Hepatocyte Growth Factor; Fibroblast-D

  • AA Sequence

    QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

  • Predicted Molecular Mass

    79.7 kDa

  • Molecular Weight

    Approximately 31-35 (β chain) & 59-68 (α chain) & 90-96 (α+β chain)kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.7597 ng/mL, corresponding to a specific activity is 1.316×106 U/mg.

  • HGF Protein, Human (HEK293) Product Image
    1/16
  • Experimental Validation Results for HGF Protein, Human (HEK293)
  • Experimental Validation Results for HGF Protein, Human (HEK293)
  • HGF Protein, Human (HEK293) Product Image