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IL-31R alpha Protein, Human (HEK293, His)

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Based on 1 Customer Validation

IL-31R alpha protein binds to IgM heavy and light chains and helps IgM assembly and secretion. It can act as a molecular chaperone or an oxidoreductase, exhibiting low oxidoreductase activity. IL-31R alpha Protein, Human (HEK293, His) is the recombinant human-derived IL-31R alpha protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for IL-31R alpha Protein, Human (HEK293, His)
  • IL-31R alpha Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

IL-31R alpha protein binds to IgM heavy and light chains and helps IgM assembly and secretion. It can act as a molecular chaperone or an oxidoreductase, exhibiting low oxidoreductase activity. IL-31R alpha Protein, Human (HEK293, His) is the recombinant human-derived IL-31R alpha protein, expressed by HEK293 , with C-His labeled tag.

Background

MZB1/PERP1 associates with immunoglobulin M (IgM) heavy and light chains, facilitating IgM assembly and secretion. It may function as a molecular chaperone or oxidoreductase, as it exhibits a low level of oxidoreductase activity. Additionally, isoform 2 may play a role in apoptosis regulation. MZB1/PERP1 contributes to the diversification of peripheral B-cell functions by modulating Ca(2+) stores, antibody secretion, and integrin activation. Furthermore, it acts as a hormone-regulated adipokine/pro-inflammatory cytokine implicated in chronic inflammation, affecting cellular expansion and attenuating insulin response in adipocytes, potentially playing a role in the onset of insulin resistance.

Verified Bioactivity

1.Immobilized human IL31RA-His at 2 μg/mL (100 μl/well) can bind biotinylated cynomolgus IL31h , The EC50 of biotinylated cynomolgus IL31h is 15-60 ng/mL.
2.Immobilized Recombinant Human Interleukin-31 receptor A / IL31RA Protein (His Tag) at 2 μg/mL (100 μL/well) can bind IL31-His/Biotin , the EC50 is 12-36 ng/mL.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Nemolizumab (HY-P99162) on AHC2 biosensor, can bind IL-31R alpha Protein, Human (HEK293, His) with an affinity constant of 1.825E-09 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q8NI17-1/EAW54932.1 (A20-S516)

  • Gene ID
    133396  [NCBI]
  • Molecular Construction
    • N-term
    • IL-31Rα (A20-S516)
      Accession # Q8NI17/EAW54932.1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    IL31RA; Cytokine Receptor-Like 3; Interleukin 31 Receptor A; IL-31R Subunit Alpha; GLM-R; HGLM-R; IL-31RA; GPL; CRL3; Soluble Type I Cytokine Receptor CRL3; Interleukin-31 Receptor Subunit Alpha; Class I Cytokine Receptor; Gp130-Like Monocyte Receptor; Gp

  • AA Sequence

    ALPAKPENISCVYYYRKNLTCTWSPGKETSYTQYTVKRTYAFGEKHDNCTTNSSTSENRASCSFFLPRITIPDNYTIEVEAENGDGVIKSHMTYWRLENIAKTEPPKIFRVKPVLGIKRMIQIEWIKPELAPVSSDLKYTLRFRTVNSTSWMEVNFAKNRKDKNQTYNLTGLQPFTEYVIALRCAVKESKFWSDWSQEKMGMTEEEAPCGLELWRVLKPAEADGRRPVRLLWKKARGAPVLEKTLGYNIWYYPESNTNLTETMNTTNQQLELHLGGESFWVSMISYNSLGKSPVATLRIPAIQEKSFQCIEVMQACVAEDQLVVKWQSSALDVNTWMIEWFPDVDSEPTTLSWESVSQATNWTIQQDKLKPFWCYNISVYPMLHDKVGEPYSIQAYAKEGVPSEGPETKVENIGVKTVTITWKEIPKSERKGIICNYTIFYQAEGGKGFSKTVNSSILQYGLESLKRKTSYIVQVMASTSAGGTNGTSINFKTLSFS

  • Predicted Molecular Mass

    58.2 kDa

  • Glycosylation

    Yes

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Loaded Nemolizumab (HY-P99162) on AHC2 biosensor, can bind IL-31R alpha Protein, Human (HEK293, His) with an affinity constant of 1.825E-09 M as determined in BLI assay.

  • IL-31R alpha Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IL-31R alpha Protein, Human (HEK293, His)
  • IL-31R alpha Protein, Human (HEK293, His) Product Image