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PD-L1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

PD-L1 Protein is an important immunoregulatory molecule and a member of the B7 superfamily. As the primary ligand of PD-1, PD-L1 Protein can bind to PD-1 to inhibit T-cell activation, proliferation, and cytokine secretion, and induce T-cell apoptosis. PD-L1 Protein plays a key role in tumor immune escape and immune homeostasis regulation. PD-L1 Protein, Human (HEK293, His) is a recombinant PD-L1 protein expressed by HEK293 with a C-His His tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PD-L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-L1 Protein, Human (HEK293, His)
  • PD-L1 Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

PD-L1 Protein is an important immunoregulatory molecule and a member of the B7 superfamily. As the primary ligand of PD-1, PD-L1 Protein can bind to PD-1 to inhibit T-cell activation, proliferation, and cytokine secretion, and induce T-cell apoptosis. PD-L1 Protein plays a key role in tumor immune escape and immune homeostasis regulation. PD-L1 Protein, Human (HEK293, His) is a recombinant PD-L1 protein expressed by HEK293 with a C-His His tag[1][2][3].

Background

PD-L1 is a 33 kDa type I transmembrane glycoprotein containing 290 amino acids, with Ig- and IgC domains in its extracellular region. Under normal conditions, PD-L1 maintains immune homeostasis by binding to PD-1 on the surface of T cells to inhibit excessive immune responses. In tumors, abnormally high expression of PD-L1 binds to PD-1 to suppress T cell function, thereby helping tumors evade immune surveillance. PD-L1 may also be involved in tumor angiogenesis and microenvironmental remodeling. Currently, as a core target, PD-L1 has driven the development of tumor immune checkpoint inhibitor therapy[1][2][3].

Verified Bioactivity

1. 2 µg/mL (100 µL/well) of immoblized recombinant human PD-L1-His can bind human PD-1-Fc with a linear range of 24-390 ng/mL.
2. Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 1.012 μg/mL, corresponding to a specific activity is 988.14 units/mg.
3. Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human PD-1 (HY-P7396). The ED50 for this effect is <0.5 μg/mL.
4. Immobilized Anti-Human PDL1 mAb at 2 μg/mL (100 μL/well) can bind Human PD-L1. The ED50 of Human PD-L1 is 1-10 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 1.012 μg/mL, corresponding to a specific activity is 988.14 units/mg.

  • Bioactivity - SPR

    Bioactivity - SPR

    PD-1 Protein, Human (HY-P7395) captured on CM5 Chip can bind PD-L1 Protein, Human (HEK293,His) with an affinity constant of 1.86E-7 M as determined in SPR assay (Biacore 8K).

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q9NZQ7-1 (F19-T239)

  • Gene ID
    29126  [NCBI]
  • Molecular Construction
    • N-term
    • PD-L1 (F19-T239)
      Accession # Q9NZQ7-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CD274; B7 Homolog 1; Prev. PDCD1LG1; B7-H; PD-L1; PDCD1 Ligand 1; B7H1; PDCD1L1; PDL1; HPD-L1; Programmed Cell Death 1 Ligand 1; Programmed Death Ligand 1; CD274 Antigen; ADMIO5; CD274 Molecule; B7-H1

  • AA Sequence

    FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERT

  • Molecular Weight

    Approximately 31-42 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 4% mannitol, 0.02% Tween 80, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Chemnitz JM, et al. SHP-1 and SHP-2 associate with immunoreceptor tyrosine-based switch motif of programmed death 1 upon primary human T cell stimulation, but only receptor ligation prevents T cell activation. J Immunol. 2004 Jul 15;173(2):945-54. [Content Brief]

[2]. Han Y, et al. PD-1/PD-L1 pathway: current researches in cancer. Am J Cancer Res. 2020 Mar 1;10(3):727-742. [Content Brief]

[3]. Kythreotou A, et al. PD-L1. Journal of clinical pathology, 2018, 71(3): 189-194.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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=
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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 1.012 μg/mL, corresponding to a specific activity is 988.14 units/mg.

  • 1/16

    MedChemExpress Validation
     PD-1 Protein, Human (HY-P7395) captured on CM5 Chip can bind PD-L1 Protein, Human (HEK293,His) with an affinity constant of 1.86E-7 M as determined in SPR assay (Biacore 8K).

  • PD-L1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PD-L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-L1 Protein, Human (HEK293, His)
  • PD-L1 Protein, Human (HEK293, His) Product Image