PD-L1 Protein, Rat (HEK293, His)

PD-L1 (Programmed death-ligand 1) critically regulates T cell proliferation and migration, acting as a biomarker for periodontitis and pre-eclampsia. CD274 is its human ortholog. Biased expression in the thymus (RPKM 102.9), spleen (RPKM 58.3), and other tissues emphasizes PD-L1's centrality in immune regulation across diverse physiological and pathological conditions. PD-L1 Protein, Rat (HEK293, His) is the recombinant rat-derived PD-L1 protein, expressed by HEK293 , with C-10*His labeled tag.

Programmed death-ligand 1 (PD-L1) plays a critical role in the positive regulation of T cell proliferation and cell migration. Situated on the cell surface, PD-L1 serves as a biomarker for conditions such as periodontitis and pre-eclampsia. Its orthologous counterpart in humans is CD274 (CD274 molecule). With biased expression observed in the thymus (RPKM 102.9), spleen (RPKM 58.3), and various other tissues, PD-L1 is central to immune regulation and holds significance in the context of diverse physiological and pathological conditions.

1. Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.1872 μg/ml in the presence of 10 μg/mL anti-CD3, corresponding to a specific activity is 5.34×10^3 units/mg.
2. Measured by its binding ability in a functional ELISA. When Recombinant Rat PD-1 is present at 1 μg/mL, can bind Recombinant Rat PD-L1. The ED₅₀ for this effect is 0.526 μg/mL.

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  Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED₅₀ this effect is 0.1872 μg/mL in the presence of 10 μg/mL anti-CD3, corresponding to a specific activity is 5.34×10³ units/mg.

Rat

HEK293

C-10*His

NP_001178883.1 (A18-T238)

499342  [NCBI]

Partial

AFTITAPKDLYVVEYGSNVTMECRFPVEQKLDLLALVVYWEKEDKEVIQFVEGEEDLKPQHSSFRGRAFLPKDQLLKGNAVLQITDVKLQDAGVYCCMISYGGADYKRITLKVNAPYRKINQRISMDPATSEHELMCQAEGYPEAEVIWTNSDHQSLSGETTVTTSQTEEKLLNVTSVLRVNATANDVFHCTFWRVHSGENHTAELIIPELPVPRLPHNRT

Approximately 41.79 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.