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RIOK2 Protein, Human (sf9, His, GST)

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Based on 1 publication(s) in Google Scholar

RIOK2 is an important serine/threonine protein kinase that is critical in the final cytoplasmic maturation phase of the 40S ribosomal subunit. It promotes the export of 40S preribosomal particles from the nucleus to the cytoplasm, which is essential for ribosome biogenesis. RIOK2 Protein, Human (sf9, His, GST) is the recombinant human-derived RIOK2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • RIOK2 Protein, Human (sf9, His, GST) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

RIOK2 is an important serine/threonine protein kinase that is critical in the final cytoplasmic maturation phase of the 40S ribosomal subunit. It promotes the export of 40S preribosomal particles from the nucleus to the cytoplasm, which is essential for ribosome biogenesis. RIOK2 Protein, Human (sf9, His, GST) is the recombinant human-derived RIOK2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

Background

RIOK2, a serine/threonine-protein kinase, plays a pivotal role in the final stages of cytoplasmic maturation of the 40S ribosomal subunit. Its involvement extends to the export of pre-40S ribosome particles from the nucleus to the cytoplasm, a crucial step in ribosomal biogenesis. The kinase activity of RIOK2 is essential for orchestrating the release of NOB1, PNO1, and LTV1 from the late pre-40S subunit and facilitating the processing of 18S-E pre-rRNA into mature 18S rRNA. Beyond its role in ribosome maturation, RIOK2 exhibits regulatory functions in the cell cycle, specifically influencing the timing of the metaphase-anaphase transition during mitotic progression. This regulatory role is likely modulated by phosphorylation, possibly by PLK1, highlighting the multifaceted contributions of RIOK2 to cellular processes.

Verified Bioactivity

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-8*His;N-GST
  • Accession

    Q9BVS4 (G2-E552)

  • Gene ID
    55781
  • Molecular Construction
    • N-term
    • 8*His-GST
    • RIOK2 (G2-E552)
      Accession # Q9BVS4
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    RIOK2; FLJ11159; RIO Kinase 2; RIO2; Serine/Threonine-Protein Kinase RIO2; RIO Kinase 2 (Yeast)

  • AA Sequence

    GKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE

  • Predicted Molecular Mass

    90.8 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 5 mM DTT, 0.1M trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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