RIOK2 Protein, Human (sf9, His, GST)

RIOK2 is an important serine/threonine protein kinase that is critical in the final cytoplasmic maturation phase of the 40S ribosomal subunit. It promotes the export of 40S preribosomal particles from the nucleus to the cytoplasm, which is essential for ribosome biogenesis. RIOK2 Protein, Human (sf9, His, GST) is the recombinant human-derived RIOK2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

RIOK2, a serine/threonine-protein kinase, plays a pivotal role in the final stages of cytoplasmic maturation of the 40S ribosomal subunit. Its involvement extends to the export of pre-40S ribosome particles from the nucleus to the cytoplasm, a crucial step in ribosomal biogenesis. The kinase activity of RIOK2 is essential for orchestrating the release of NOB1, PNO1, and LTV1 from the late pre-40S subunit and facilitating the processing of 18S-E pre-rRNA into mature 18S rRNA. Beyond its role in ribosome maturation, RIOK2 exhibits regulatory functions in the cell cycle, specifically influencing the timing of the metaphase-anaphase transition during mitotic progression. This regulatory role is likely modulated by phosphorylation, possibly by PLK1, highlighting the multifaceted contributions of RIOK2 to cellular processes.

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-8*His;N-GST

Q9BVS4 (G2-E552)

55781

Partial

GKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE

Approximately 90.8 KDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 5 mM DTT, 0.1M trehalose.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.