VEGFR-3/FLT4 Protein, Human (HEK293, His)

VEGFR-2 protein is an important tyrosine protein kinase receptor that serves as a cell surface receptor for VEGFA, VEGFC and VEGFD to regulate angiogenesis, blood vessel development and embryonic hematopoiesis. It enhances endothelial cell proliferation, survival, migration and differentiation. VEGFR-3/FLT4 Protein, Human (HEK293, His) is the recombinant human-derived VEGFR-3/FLT4 protein, expressed by HEK293 , with C-His labeled tag.

VEGFR-2 protein is a tyrosine-protein kinase receptor that plays a crucial role in regulating various processes including angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. It acts as a cell-surface receptor for VEGFA, VEGFC, and VEGFD, promoting the proliferation, survival, migration, and differentiation of endothelial cells. Additionally, isoforms lacking a transmembrane domain, such as isoform 2, act as decoy receptors for VEGFA, VEGFC, and/or VEGFD, serving as negative regulators of lymphangiogenesis by inhibiting the binding of these growth factors to FLT4. VEGFR-2 also forms heterodimers with FLT1 and FLT4, modulating their signaling. Activation of isoform 1 by vascular growth factors triggers multiple signaling cascades, including the activation of PLCG1, resulting in the production of diacylglycerol and inositol 1,4,5-trisphosphate, as well as the activation of protein kinase C. It also activates MAPK1/ERK2, MAPK3/ERK1, and AKT1 signaling pathways, leading to various cellular responses. Moreover, VEGFR-2 phosphorylates PIK3R1, facilitating the activation of phosphatidylinositol 3-kinase, and promotes actin cytoskeleton reorganization and activation of PTK2/FAK1. Its role in NOS2 and NOS3 induction leads to the production of nitric oxide (NO) by endothelial cells. Additionally, VEGFR-2 phosphorylates PLCG1 and promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1, and SRC, further contributing to its diverse cellular functions.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA.
3.Immobilized human VEGF-C at 10 μg/mL (100 μl/wel) can bind human VEGFR3-his The EC₅₀ of human VEGFR3-his is 0.011 μg/mL.

Human

HEK293

C-His

P35916-2/NP_002011.2 (Y25-I776)

2324  [NCBI]

Partial

YSMTPPTLNITEESHVIDTGDSLSISCRGQHPLEWAWPGAQEAPATGDKDSEDTGVVRDCEGTDARPYCKVLLLHEVHANDTGSYVCYYKYIKARIEGTTAASSYVFVRDFEQPFINKPDTLLVNRKDAMWVPCLVSIPGLNVTLRSQSSVLWPDGQEVVWDDRRGMLVSTPLLHDALYLQCETTWGDQDFLSNPFLVHITGNELYDIQLLPRKSLELLVGEKLVLNCTVWAEFNSGVTFDWDYPGKQAERGKWVPERRSQQTHTELSSILTIHNVSQHDLGSYVCKANNGIQRFRESTEVIVHENPFISVEWLKGPILEATAGDELVKLPVKLAAYPPPEFQWYKDGKALSGRHSPHALVLKEVTEASTGTYTLALWNSAAGLRRNISLELVVNVPPQIHEKEASSPSIYSRHSRQALTCTAYGVPLPLSIQWHWRPWTPCKMFAQRSLRRRQQQDLMPQCRDWRAVTTQDAVNPIESLDTWTEFVEGKNKTVSKLVIQNANVSAMYKCVVSNKVGQDERLIYFYVTTIPDGFTIESKPSEELLEGQPVLLSCQADSYKYEHLRWYRLNLSTLHDAHGNPLLLDCKNVHLFATPLAASLEEVAPGARHATLSLSIPRVAPEHEGHYVCEVQDRRSHDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMQCLVAGAHAPSIVWYKDERLLEEKSGVDLADSNQKLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSMEI

Approximately 130 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS. pH 7.4.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.