Resazurin sodium, indicator
Based on 1 Customer Validation
Resazurin (Diazoresorcinol) sodium, indicator is a non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin sodium, indicator is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin sodium, indicator can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal).
For research use only. We do not sell to patients.
- Purity: 98.06%
- CAS No.: 62758-13-8
- Formula: C12H6NNaO4
- Molecular Weight:251.17
-
Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-111391
Resazurin sodium
-
HY-29350
Polyglycerol-polyricinoleate
-
HY-15924
Thiazolyl Blue
-
HY-K1070
Apoptosis and Necrosis Assay Kit
-
HY-111391R
Resazurin sodium (Standard)
-
HY-D0718
Nile Red
-
HY-DY1051
Resazurin sodium (solution)
-
HY-DY1008
Nile Red (solution)
-
HY-12591A
D-Luciferin
-
HY-12591B
D-Luciferin potassium
-
HY-12591
D-Luciferin sodium
-
HY-D0815
Propidium Iodide
Biological Activity
Resazurin sodium, indicator is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin. No viable bacteria are detected 24 h post-inoculation following inclusion of Resazurin sodium, indicator in TSBc cultures of F. tularensis LVS at the concentration of 44 μM. Lowering the Resazurin sodium, indicator concentration to as little as 4.4 μM still results in a 10-fold reduction in viable F. tularensis LVS compare to growth medium alone. Both Resazurin sodium, indicator treatments result in a significant decrease in viable F. tularensis LVS bacteria over 22 h. Treatment with Resazurin sodium, indicator significantly reduces the number of viable F. tularensis LVS bacteria in HEK293 cells 22 h post-infection[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solution preparation[2][3]
1.1 Preparation of stock solution
Solvent: DMSO or ddH2O
Concentration: 1 mg/mL (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1 µg/mL with PBS or serum-free medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell viability
1. Defrost the Resazurin sodium, indicator solution in a 37°C water bath.
2. Place the cells on a 96-well plate, and washed by PBS (avoid light).
3. Remove the PBS wash, then add 500 μL (1 μg/mL) of Resazurin sodium, indicator solution.
4. Place the plate, in the incubator for 30 min (incubation time depends on cell type and cell number).
5. Measure Resazurin sodium, indicator fluorescence (Ex=530-560 nm, Em=590 nm) using a microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 62758-13-8
-
Appearance Solid
-
Molecular Weight 251.17
-
Formula C12H6NNaO4
-
Color Brown to black
-
SMILES
O=C1C=CC2=[N+]([O-])C3=C(C=C(O[Na])C=C3)OC2=C1
-
Synonyms
Diazoresorcinol sodium, indicator
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 25 mg/mL (99.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (19.91 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93. [Content Brief]
[2]. Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60. [Content Brief]
[3]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.9814 mL | 19.9068 mL | 39.8137 mL | 99.5342 mL |
| 5 mM | 0.7963 mL | 3.9814 mL | 7.9627 mL | 19.9068 mL | |
| 10 mM | 0.3981 mL | 1.9907 mL | 3.9814 mL | 9.9534 mL | |
| 15 mM | 0.2654 mL | 1.3271 mL | 2.6542 mL | 6.6356 mL | |
| DMSO | 20 mM | 0.1991 mL | 0.9953 mL | 1.9907 mL | 4.9767 mL |
| 25 mM | 0.1593 mL | 0.7963 mL | 1.5925 mL | 3.9814 mL | |
| 30 mM | 0.1327 mL | 0.6636 mL | 1.3271 mL | 3.3178 mL | |
| 40 mM | 0.0995 mL | 0.4977 mL | 0.9953 mL | 2.4884 mL | |
| 50 mM | 0.0796 mL | 0.3981 mL | 0.7963 mL | 1.9907 mL | |
| 60 mM | 0.0664 mL | 0.3318 mL | 0.6636 mL | 1.6589 mL | |
| 80 mM | 0.0498 mL | 0.2488 mL | 0.4977 mL | 1.2442 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Resazurin sodium, indicator Related Classifications
HY-111391A Related Classifications
Powered by Bioz