RNF5 inhibitor inh-02
Based on 1 Customer Validation
RNF5 inhibitor inh-02 is a potent inhibitor of E3 ubiquitin ligase RNF5/RMA1. RNF5 inhibitor inh-02 rescues F508del-CFTR function in F508del-CFTR-expressing immortalized cells (CFBE41o⁻, EC50 = 2.6 μM; FRT, EC 50 = 2.2 μM). RNF5 inhibitor inh-02 increases LC3IIB expression and autophagic vacuole number via reducing ATG4B ubiquitylation and promotes cell motility. RNF5 inhibitor inh-02 can be used for the study of cystic fibrosis.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 324579-65-9
- Formula: C23H20N4S
- Molecular Weight:384.50
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
RNF5 inhibitor inh-02 (2-10 μM, 24 h) significantly reduces F508del-CFTR ubiquitylation and rescues F508del-CFTR activity (EC50 = 2.6 μM) in CFBE41o- cells stably expressing F508del-CFTR and HS-YFP[1].
RNF5 inhibitor inh-02 (1-10 μM, 24 h) dose-dependently rescues F508del-CFTR activity in F508del-CFTR/HS-YFP-expressing FRT cells with an EC50 of 2.2 μM, and increases CFTR-dependent transepithelial conductance[1].
RNF5 inhibitor inh-02 (5 μM, 24 h) significantly increases CFTR-mediated transepithelial conductance, equivalent short-circuit current and chloride secretion in primary bronchial epithelial cells[1].
RNF5 inhibitor inh-02 (5 μM, 24 h) reduces ATG4B ubiquitylation, increases LC3IIB expression and autophagic vacuole number in CFBE41o- cells[1].
RNF5 inhibitor inh-02 (5 μM, 24 h) promotes cell migration and accelerates scratch gap filling in CFBE41o- cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 324579-65-9
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Appearance Solid
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Molecular Weight 384.50
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Formula C23H20N4S
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Color White to yellow
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SMILES
CC1=NS/C(N1CC2=CC=CC=C2)=N\C(C3=CC=CC=C3)=N\C4=CC=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 83.33 mg/mL (216.72 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (614 KB)
- English - EN (614 KB)
- Français - FR (614 KB)
- Deutsch - DE (614 KB)
- Norwegian - NO (614 KB)
- Español - ES (614 KB)
- Swedish - SV (614 KB)
- Italian - IT (614 KB)
- Korean - KR (614 KB)
- Portuguese - PT (614 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6008 mL | 13.0039 mL | 26.0078 mL | 65.0195 mL |
| 5 mM | 0.5202 mL | 2.6008 mL | 5.2016 mL | 13.0039 mL | |
| 10 mM | 0.2601 mL | 1.3004 mL | 2.6008 mL | 6.5020 mL | |
| 15 mM | 0.1734 mL | 0.8669 mL | 1.7339 mL | 4.3346 mL | |
| 20 mM | 0.1300 mL | 0.6502 mL | 1.3004 mL | 3.2510 mL | |
| 25 mM | 0.1040 mL | 0.5202 mL | 1.0403 mL | 2.6008 mL | |
| 30 mM | 0.0867 mL | 0.4335 mL | 0.8669 mL | 2.1673 mL | |
| 40 mM | 0.0650 mL | 0.3251 mL | 0.6502 mL | 1.6255 mL | |
| 50 mM | 0.0520 mL | 0.2601 mL | 0.5202 mL | 1.3004 mL | |
| 60 mM | 0.0433 mL | 0.2167 mL | 0.4335 mL | 1.0837 mL | |
| 80 mM | 0.0325 mL | 0.1625 mL | 0.3251 mL | 0.8127 mL | |
| 100 mM | 0.0260 mL | 0.1300 mL | 0.2601 mL | 0.6502 mL |
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