2. Metabolic Enzyme/Protease Others
  3. Liposome mRNA
  4. S-Ac7-DOg

S-Ac7-DOG is a cationic lipid with biodegradability, low immunogenicity and high nucleic acid transfection capacity, which is commonly used to construct lipid nanoparticles for nucleic acid molecule delivery. S-Ac7-DOG can bind to mRNA, microRNA and self-amplifying RNA through electrostatic interaction. Lipid nanoparticles formed by S-Ac7-DOG enter cells via an energy-dependent endocytic pathway, release nucleic acid cargos, induce antigen-specific CD8+ T cell responses, promote the generation of precursor memory T cells, and regulate neuroinflammatory pathways. S-Ac7-DOG can be used in the research of retinal diseases, neuroinflammation and cancer.

For research use only. We do not sell to patients.

S-Ac7-DOg

S-Ac7-DOg Chemical Structure

CAS No. : 2796227-17-1

Size Stock
5 mg Check price and availability
10 mg Check price and availability
25 mg Check price and availability
50 mg Check price and availability
100 mg Check price and availability

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

S-Ac7-DOg Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

S-Ac7-DOG is a cationic lipid with biodegradability, low immunogenicity and high nucleic acid transfection capacity, which is commonly used to construct lipid nanoparticles for nucleic acid molecule delivery. S-Ac7-DOG can bind to mRNA, microRNA and self-amplifying RNA through electrostatic interaction. Lipid nanoparticles formed by S-Ac7-DOG enter cells via an energy-dependent endocytic pathway, release nucleic acid cargos, induce antigen-specific CD8+ T cell responses, promote the generation of precursor memory T cells, and regulate neuroinflammatory pathways. S-Ac7-DOG can be used in the research of retinal diseases, neuroinflammation and cancer[1][2][3][4].

In Vitro

S-Ac7-DOg (5 h; stored at 4 °C)-loaded mRNA and saRNA LNPs exhibit consistent physicochemical properties (size, PDI, zeta potential, encapsulation efficiency) suitable for in vitro retinal delivery[1].
S-Ac7-DOg LNPs (0.5 μg; 24 h) mediate efficient cellular uptake and high transfection efficiency in ARPE-19 and MIO-M1 cells for mRNA delivery, with mRNA cargo inducing more eGFP-positive cells than saRNA cargo[1].
S-Ac7-DOg-based LNP (5-80 nM siRNA; 24 h) efficiently silences luciferase activity in Luc-BV2 mouse microglial cells, with comparable performance to other leading formulations at a 40 nM siRNA dose[2].
S-Ac7-DOg-based LNP (5-80 nM siRNA; 24 h) exhibits no cytotoxicity in BV2 mouse microglial cells at siRNA concentrations ranging from 5 to 80 nM after 24 h incubation[2].
S-Ac7-DOg-based LNP (40 nM miR-124; 24 h) achieves the highest miR-124 transfection efficiency in BV2 mouse microglial cells after 24 h incubation at a 40 nM miRNA dose[2].
S-Ac7-DOg-based LNP (40 nM Cy5-miR-124; 1-6 h) are internalized by BV2 mouse microglial cells via an energy-dependent, cholesterol-requiring endocytic pathway, and successfully escape from endosomes over time[2].
S-Ac7-DOG LNPs (50-200 ng mRNA; 24 h) mediate 10- to 100-fold higher eGFP expression than MC3 LNPs in HEK293T, MC38, and CT26 cells, while maintaining low cytotoxicity[3].
S-Ac7-DOG LNPs (50-200 ng mRNA; 24 h) exhibit significantly higher transfection efficiency than MC3 LNPs in CT26 cells, with no difference in cellular LNP association[3].
S-Ac7-DOG LNPs induce earlier onset of human RBC lysis at acidic pH values relative to MC3 LNPs, indicating enhanced membrane rupture activity at higher endosomal pH[3].
S-Ac7-DOG LNPs undergo more robust disassembly in the presence of anionic DOPS:DOPC liposomes at acidic pH values relative to MC3 LNPs, indicating enhanced membrane interaction capacity[3].
S-Ac7-DOG modified eLNPs can effectively bind mRNA and maintain colloidal stability and mRNA binding ability after storage.[4].
S-Ac7-DOG eLNP+ mRNA showed no detectable cytotoxicity and completely protected the mRNA from RNase degradation; cellular uptake was also higher[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

S-Ac7-DOg Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: BV2 mouse microglial cells
Concentration: 5, 10, 20, 40, 80 nM siRNA
Incubation Time: 24 h
Result: Did not reduce BV2 cell viability at any tested siRNA concentration.
Showed no dose-dependent cytotoxicity.

Real Time qPCR[2]

Cell Line: BV2 mouse microglial cells
Concentration: 40 nM miR-124
Incubation Time: 24 h
Result: Resulted in the highest intracellular miR-124 levels.
Achieved significantly elevated miR-124 levels relative to miR-negative control LNP.

Cell Cytotoxicity Assay[3]

Cell Line: HEK293T human embryonic kidney cells, MC38 mouse colon cancer cells, CT26 mouse colon cancer cells
Concentration: 50 ng mRNA; 200 ng mRNA
Incubation Time: 24 h
Result: Induced 10- to 100-fold higher eGFP fluorescence compared to benchmark MC3 LNPs across all three cell lines and both mRNA doses.
Displayed low cytotoxicity relative to other tested lipids.
In Vivo

S-Ac7-DOg (1 μg mRNA, 0.2 μg saRNA; intravitreal injection; single dose) induces only extremely mild or even no local inflammatory response in mice[1].
S-Ac7-DOg (5 μg; intramuscular injection; single administration) achieves higher local miR-124 levels in the muscles of healthy mice than MC3-LNP, and induces lower expression levels of proinflammatory cytokines[2].
S-Ac7-DOG LNPs (5 μg mRNA; intramuscular injection; single administration) mediate sustained, local mRNA expression at the injection site, while reducing off-target liver distribution, resulting in higher muscle-to-systemic bioluminescence ratios and higher spleen-to-liver bioluminescence ratios[3].
S-Ac7-DOG LNPs (5 μg mRNA; intramuscular injection; single administration) exhibit low reactogenicity and unique cell tropism compared with MC3 LNPs[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

968.48

Formula

C53H97N3O8S2
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)CNC(OCCSSCCOC(NCCN1CCCCCC1)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (103.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (51.63 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0325 mL 5.1627 mL 10.3255 mL
5 mM 0.2065 mL 1.0325 mL 2.0651 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 1.0325 mL 5.1627 mL 10.3255 mL 25.8136 mL
5 mM 0.2065 mL 1.0325 mL 2.0651 mL 5.1627 mL
10 mM 0.1033 mL 0.5163 mL 1.0325 mL 2.5814 mL
15 mM 0.0688 mL 0.3442 mL 0.6884 mL 1.7209 mL
20 mM 0.0516 mL 0.2581 mL 0.5163 mL 1.2907 mL
25 mM 0.0413 mL 0.2065 mL 0.4130 mL 1.0325 mL
30 mM 0.0344 mL 0.1721 mL 0.3442 mL 0.8605 mL
40 mM 0.0258 mL 0.1291 mL 0.2581 mL 0.6453 mL
50 mM 0.0207 mL 0.1033 mL 0.2065 mL 0.5163 mL
DMSO 60 mM 0.0172 mL 0.0860 mL 0.1721 mL 0.4302 mL
80 mM 0.0129 mL 0.0645 mL 0.1291 mL 0.3227 mL
100 mM 0.0103 mL 0.0516 mL 0.1033 mL 0.2581 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

S-Ac7-DOg Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

S-Ac7-DOg2796227-17-1LiposomemRNAmessenger RNAHEK293T cellsretinal diseasesARPE-19 cellsMC38 cellsMIO-M1 cellsBV2 mouse microglial cellsmiRNAsaRNACT26 cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
S-Ac7-DOg
Cat. No.:
HY-177531
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.