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0.5M EDTA Solution, pH 8.0

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By Targets:	Biochemical Assay Reagents (7) Environmental Pollutants (1) Fluorescent Dye (2)

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Fluorescent Dyes

Biochemical Assay Reagents

MCE Kits

Targets Recommended: HIF/HIF Prolyl-Hydroxylase

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-107921

EDTA Calcium disodium,98% EDTA calcium disodium salt,98%; Adsorbonac,98%	Environmental Pollutants Biochemical Assay Reagents	Others
EDTA Calcium disodium, 98% (EDTA calcium disodium salt, 98%; Adsorbonac, 98%) is a chelating agent for calcium and other metal ions, preventing them from participating in chemical reactions. It can effectively remove metal ions from solutions, preventing them from interfering with experimental processes.

HY-Y0682I

0.5M Ethylenediaminetetraacetic acid Solution, pH 8.0 0.5M EDTA Solution, pH 8.0	Biochemical Assay Reagents	Others
0.5M Ethylenediaminetetraacetic acid Solution, pH 8.0 (0.5M EDTA Solution, pH 8.0) is a commonly used molecular biology reagent used for chelating magnesium ions, etc. This product is prepared using EDTA-Na2 and the pH value is adjusted to 8.0 with NaOH.

HY-D1895A

3,5-DiBr-PAESA sodium	Biochemical Assay Reagents	Others
3,5-DiBr-PAESA sodium is a water-soluble pyridylazo-based highly sensitive chromogenic reagent. With the assistance of EDTA for masking copper and bromide ions for dissociating silver chelates, 3,5-DiBr-PAESA sodium enables accurate determination of sub-milligram level silver ions in strongly acidic high-copper systems via flow injection spectrophotometry .

HY-W151206

Hydroxy naphthol blue disodium	Fluorescent Dye	Others
Hydroxy naphthol blue disodium is an azo dye compound commonly used as an acid-base indicator (λmax = 650 nm). Within the pH range of 12 to 13, the indicator solution of Hydroxy naphthol blue disodium appears pale red in the presence of calcium ions and dark blue in the presence of disodium EDTA .

HY-D1895

3,5-DiBr-PAESA	Biochemical Assay Reagents	Others
3,5-DiBr-PAESA is a water-soluble pyridylazo-based highly sensitive chromogenic reagent. With the assistance of EDTA for masking copper and bromide ions for dissociating silver chelates, 3,5-DiBr-PAESA enables accurate determination of sub-milligram level silver ions in strongly acidic high-copper systems via flow injection spectrophotometry .

HY-W243460

EDTA copper(II) disodium salt, 97%	Biochemical Assay Reagents	Others
EDTA copper (II) disodium salt, 97% is a negatively charged copper (II)-ethylenediaminetetraacetic acid chelate and also an adsorption substrate. EDTA copper (II) disodium salt, 97% forms amide bonds with chitosan amino groups in weakly acidic (pH 3-5) solutions, while it forms the CuEDTA (OH) ³⁻ hydroxyl complex in strongly alkaline (pH > 12) solutions. EDTA copper (II) disodium salt, 97% can be adsorbed onto granular activated carbon, with electrostatic interactions dominating its pH-dependent adsorption behavior. EDTA copper (II) disodium salt, 97% can be used to eliminate the inhibition of enzyme-catalyzed reactions caused by trace heavy metals .

HY-W133934A

EDTA magnesium disodium,99%	Biochemical Assay Reagents	Others
EDTA magnesium disodium, 99% is a chelating agent with the ability to effectively bind metal ions (including calcium, magnesium, and iron) and remove them from solution.

HY-182668

EDTA Antigen Retrieval Solution, 50×	Biochemical Assay Reagents	Others
EDTA Antigen Retrieval Solution, 50× is a commonly used antigen retrieval solution that can effectively remove cross-links between proteins caused by aldehyde fixation reagents, fully exposing antigenic epitopes in samples such as paraffin sections. It can be used for antigen retrieval in samples such as paraffin sections and frozen sections after fixation with paraformaldehyde, formaldehyde, or other aldehyde reagents.

HY-DY1091

Calcein (solution) Fluorexon (Solution)	Fluorescent Dye	Others
Calcein (solution) (Fluorexon (solution)) is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems . Solvent and concentration: DMSO: 2 mM The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.	Product Name	Type

HY-D1895A

3,5-DiBr-PAESA sodium	Fluorescent Dyes
3,5-DiBr-PAESA sodium is a water-soluble pyridylazo-based highly sensitive chromogenic reagent. With the assistance of EDTA for masking copper and bromide ions for dissociating silver chelates, 3,5-DiBr-PAESA sodium enables accurate determination of sub-milligram level silver ions in strongly acidic high-copper systems via flow injection spectrophotometry .

HY-W151206

Hydroxy naphthol blue disodium	Fluorescent Dyes
Hydroxy naphthol blue disodium is an azo dye compound commonly used as an acid-base indicator (λmax = 650 nm). Within the pH range of 12 to 13, the indicator solution of Hydroxy naphthol blue disodium appears pale red in the presence of calcium ions and dark blue in the presence of disodium EDTA .

HY-D1895

3,5-DiBr-PAESA	Fluorescent Dyes
3,5-DiBr-PAESA is a water-soluble pyridylazo-based highly sensitive chromogenic reagent. With the assistance of EDTA for masking copper and bromide ions for dissociating silver chelates, 3,5-DiBr-PAESA enables accurate determination of sub-milligram level silver ions in strongly acidic high-copper systems via flow injection spectrophotometry .

HY-DY1091

Calcein (solution) Fluorexon (Solution)	Fluorescent Dyes
Calcein (solution) (Fluorexon (solution)) is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems . Solvent and concentration: DMSO: 2 mM The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Calcein (solution)

Fluorexon (Solution)

Fluorescent Dyes

Calcein (solution) (Fluorexon (solution)) is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems .
Solvent and concentration: DMSO: 2 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.	Product Name	Type

HY-107921

EDTA Calcium disodium,98% EDTA calcium disodium salt,98%; Adsorbonac,98%	Biochemical Assay Reagents
EDTA Calcium disodium, 98% (EDTA calcium disodium salt, 98%; Adsorbonac, 98%) is a chelating agent for calcium and other metal ions, preventing them from participating in chemical reactions. It can effectively remove metal ions from solutions, preventing them from interfering with experimental processes.

HY-Y0682I

0.5M Ethylenediaminetetraacetic acid Solution, pH 8.0 0.5M EDTA Solution, pH 8.0	Biochemical Assay Reagents
0.5M Ethylenediaminetetraacetic acid Solution, pH 8.0 (0.5M EDTA Solution, pH 8.0) is a commonly used molecular biology reagent used for chelating magnesium ions, etc. This product is prepared using EDTA-Na2 and the pH value is adjusted to 8.0 with NaOH.

HY-W133934A

EDTA magnesium disodium,99%	Biochemical Assay Reagents
EDTA magnesium disodium, 99% is a chelating agent with the ability to effectively bind metal ions (including calcium, magnesium, and iron) and remove them from solution.

HY-182668

EDTA Antigen Retrieval Solution, 50×	Biochemical Assay Reagents
EDTA Antigen Retrieval Solution, 50× is a commonly used antigen retrieval solution that can effectively remove cross-links between proteins caused by aldehyde fixation reagents, fully exposing antigenic epitopes in samples such as paraffin sections. It can be used for antigen retrieval in samples such as paraffin sections and frozen sections after fixation with paraformaldehyde, formaldehyde, or other aldehyde reagents.

EDTA Antigen Retrieval Solution, 50×

Biochemical Assay Reagents

EDTA Antigen Retrieval Solution, 50× is a commonly used antigen retrieval solution that can effectively remove cross-links between proteins caused by aldehyde fixation reagents, fully exposing antigenic epitopes in samples such as paraffin sections. It can be used for antigen retrieval in samples such as paraffin sections and frozen sections after fixation with paraformaldehyde, formaldehyde, or other aldehyde reagents.

Cat. No.	Product Name

HY-K3009

0.25% Trypsin-EDTA (1×), no phenol red 5 Publications Verification
MCE 0.25% Trypsin-EDTA (1×), Phenol Red-Free, is prepared by dissolving trypsin powder (a mixture of proteases from porcine pancreas, irradiated and sterilized) and EDTA in a calcium- and magnesium-free balanced salt solution. It is filter-sterilized and can be directly used for the dissociation of cultured cells and tissues. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

0.25% Trypsin-EDTA (1×), no phenol red

5 Publications Verification

MCE 0.25% Trypsin-EDTA (1×), Phenol Red-Free, is prepared by dissolving trypsin powder (a mixture of proteases from porcine pancreas, irradiated and sterilized) and EDTA in a calcium- and magnesium-free balanced salt solution. It is filter-sterilized and can be directly used for the dissociation of cultured cells and tissues. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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