Hydroxy naphthol blue disodium
Based on 1 Customer Validation
Hydroxy naphthol blue disodium is an azo dye compound commonly used as an acid-base indicator (λmax = 650 nm). Within the pH range of 12 to 13, the indicator solution of Hydroxy naphthol blue disodium appears pale red in the presence of calcium ions and dark blue in the presence of disodium EDTA.
For research use only. We do not sell to patients.
- CAS No.: 165660-27-5
- Formula: C20H12N2Na2O11S3
- Molecular Weight:598.49
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Hydroxy naphthol blue disodium (120 μM; 1 h at 63°C) enables sensitive, visual, and stable colorimetric detection of λ DNA via LAMP (down to 160 copies/tube) without inhibiting amplification, supporting high-throughput, low-contamination nucleic acid detection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 165660-27-5
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Appearance Solid
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Molecular Weight 598.49
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Formula C20H12N2Na2O11S3
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Color Brown to black
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SMILES
O=C1C=C(S(=O)(O)=O)C2=CC=CC=C2/C1=N/NC3=C(O)C(S(=O)(O[Na])=O)=CC4=CC(S(=O)(O[Na])=O)=CC=C43
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 25 mg/mL (41.77 mM; Need ultrasonic)
DMSO : 5.83 mg/mL (9.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.6709 mL | 8.3544 mL | 16.7087 mL | 41.7718 mL |
| 5 mM | 0.3342 mL | 1.6709 mL | 3.3417 mL | 8.3544 mL | |
| H2O | 10 mM | 0.1671 mL | 0.8354 mL | 1.6709 mL | 4.1772 mL |
| 15 mM | 0.1114 mL | 0.5570 mL | 1.1139 mL | 2.7848 mL | |
| 20 mM | 0.0835 mL | 0.4177 mL | 0.8354 mL | 2.0886 mL | |
| 25 mM | 0.0668 mL | 0.3342 mL | 0.6683 mL | 1.6709 mL | |
| 30 mM | 0.0557 mL | 0.2785 mL | 0.5570 mL | 1.3924 mL | |
| 40 mM | 0.0418 mL | 0.2089 mL | 0.4177 mL | 1.0443 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Hydroxy naphthol blue disodium Related Classifications
HY-W151206 Related Classifications
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