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Cat. No.: HY-161385

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Topoisomerase I inhibitor 16

Topoisomerase I inhibitor 21 (Compound 3e) is an inhibitor for Topoisomerase I through stabilization of enzyme-DNA complex. Topoisomerase I inhibitor 21 exhibits antiproliferative activity in 39 human cancer cells (JFCR39) with mean GI₅₀ 39 nM.

For research use only. We do not sell to patients.

CAS No.: 3036181-84-4
Formula: C₂₇H₂₇N₃O₄
Molecular Weight:457.52

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

Topoisomerase I inhibitor 16 Chemical Structure

CAS No.: 3036181-84-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Topoisomerase I

All Topoisomerase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	49 nM Compound: 3e	Growth inhibition of human A549 cells assessed as reduction in cell viability Growth inhibition of human A549 cells assessed as reduction in cell viability	[PMID: 38518997]
ACHN	GI₅₀	<10 nM Compound: 3e	Growth inhibition of human ACHN cells assessed as reduction in cell viability Growth inhibition of human ACHN cells assessed as reduction in cell viability	[PMID: 38518997]
DU-145	GI₅₀	<10 nM Compound: 3e	Growth inhibition of human DU-145 cells assessed as reduction in cell viability Growth inhibition of human DU-145 cells assessed as reduction in cell viability	[PMID: 38518997]
HCT-116	GI₅₀	<10 nM Compound: 3e	Growth inhibition of human HCT-116 cells assessed as reduction in cell viability Growth inhibition of human HCT-116 cells assessed as reduction in cell viability	[PMID: 38518997]
LOX IMVI	GI₅₀	<10 nM Compound: 3e	Growth inhibition of human LOX IMVI cells assessed as reduction in cell viability Growth inhibition of human LOX IMVI cells assessed as reduction in cell viability	[PMID: 38518997]
MCF7	GI₅₀	<10 nM Compound: 3e	Growth inhibition of human MCF7 cells assessed as reduction in cell viability Growth inhibition of human MCF7 cells assessed as reduction in cell viability	[PMID: 38518997]
MKN-28	GI₅₀	19 nM Compound: 3e	Growth inhibition of human MKN-28 cells assessed as reduction in cell viability Growth inhibition of human MKN-28 cells assessed as reduction in cell viability	[PMID: 38518997]
SK-OV-3	GI₅₀	18 nM Compound: 3e	Growth inhibition of human SK-OV-3 cells assessed as reduction in cell viability Growth inhibition of human SK-OV-3 cells assessed as reduction in cell viability	[PMID: 38518997]
U-251	GI₅₀	<10 nM Compound: 3e	Growth inhibition of human U-251 cells assessed as reduction in cell viability Growth inhibition of human U-251 cells assessed as reduction in cell viability	[PMID: 38518997]

In Vitro

Topoisomerase I inhibitor 21 inhibits proliferations of cancer cells MCF7, U251, HCT-166, A549, LOX-IMVI, SK-OV-3, ACHN, MKN28 and DU145, with EC₅₀s <50 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 3036181-84-4
Molecular Weight 457.52
Formula C₂₇H₂₇N₃O₄
SMILES

OC1=CC2=C(C=C1)C3=C(C(N=CC4=C5C=C(OC)C(O)=C4)=C5N3CCCN6CCOCC6)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sakai K, et al., Design, synthesis, and biological evaluation of novel benzo[6,7]indolo[3,4-c]isoquinolines as anticancer agents with topoisomerase I inhibition. Bioorg Med Chem Lett. 2024 Mar 20:129710. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Topoisomerase I inhibitor 16

All Topoisomerase Isoforms