UNC9994 hydrochloride
Based on 3 publication(s) in Google Scholar
UNC9994 hydrochloride is a functionally selective, β-arrestin–biased dopamine D2 receptor (D2R) agonist that selectively activates β-arrestin recruitment and signaling. UNC9994 hydrochloride shows a binding affinity with a Ki of 79 nM for D2R. UNC9994 hydrochloride is also an antagonist of Gi-regulated cAMP production and partial agonist for D2R/β-arrestin-2 interactions. UNC9994 hydrochloride shows antipsychotic-like activity.
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- Pureté : 99.50%
- CAS No.: 2108826-33-9
- Formule: C21H23Cl3N2OS
- Masse moléculaire:457.84
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) UNC9994 hydrochloride
MoreVoir tous les produits spécifiques à Isoform Dopamine Receptor
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More
Activité biologique
Description
IC50 & Target
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D4 Receptor 138 nM (Ki) |
D3 Receptor 17 nM (Ki) |
D2 Receptor 79 nM (Ki) |
D1 Receptor 4000 nM (Ki) |
5-HT1A Receptor 26 nM (Ki) |
5-HT2C Receptor 512 nM (Ki) |
5-HT2A Receptor 140 nM (Ki) |
5-HT2B Receptor 25 nM (Ki) |
In Vitro
UNC9994 hydrochloride induces D2-mediated β-arrestin-2 translocation with an EC50s of 6.1 nM and 448 nM in Tango assay and DiscoveRx assay, respectively[1].
UNC9994 hydrochloride is an antagonist at 5HT2A and 5HT2B and an agonist at 5HT2C and 5HT1A[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J wild-type and β-arrestin-2 knockout mice[1]
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Dosage:2.0 mg/kg, followed 30 min later with 6 mg/kg phencyclidine (PCP, i.p.)
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Administration:IP, once
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Result:Markedly inhibited PCP-induced hyperlocomotion in wild-type mice and the activity was completely abolished in β-arrestin-2 knockout mice.
Chemical Information
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CAS No. 2108826-33-9
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Appearance Solid
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Masse moléculaire 457.84
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Formule C21H23Cl3N2OS
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Color White to off-white
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SMILES
ClC1=C(Cl)C(C2CCN(CCCOC3=CC=C(SC=N4)C4=C3)CC2)=CC=C1.Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Theranostics
Morphine- and foot shock-responsive neuronal ensembles in the VTA possess different connectivity and biased GPCR signaling pathway. [Abstract]2024 Jan 12;14(3):1126-1146. PMID: 38250036 -
Acta Pharmacol Sin
G protein-dependent dopamine D2 receptor signaling mediates cocaine-primed reinstatement. [Abstract]2026 Jan 14. PMID: 41535707 -
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (218.42 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.46 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.46 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1842 mL | 10.9208 mL | 21.8417 mL | 54.6042 mL |
| 5 mM | 0.4368 mL | 2.1842 mL | 4.3683 mL | 10.9208 mL | |
| 10 mM | 0.2184 mL | 1.0921 mL | 2.1842 mL | 5.4604 mL | |
| 15 mM | 0.1456 mL | 0.7281 mL | 1.4561 mL | 3.6403 mL | |
| 20 mM | 0.1092 mL | 0.5460 mL | 1.0921 mL | 2.7302 mL | |
| 25 mM | 0.0874 mL | 0.4368 mL | 0.8737 mL | 2.1842 mL | |
| 30 mM | 0.0728 mL | 0.3640 mL | 0.7281 mL | 1.8201 mL | |
| 40 mM | 0.0546 mL | 0.2730 mL | 0.5460 mL | 1.3651 mL | |
| 50 mM | 0.0437 mL | 0.2184 mL | 0.4368 mL | 1.0921 mL | |
| 60 mM | 0.0364 mL | 0.1820 mL | 0.3640 mL | 0.9101 mL | |
| 80 mM | 0.0273 mL | 0.1365 mL | 0.2730 mL | 0.6826 mL | |
| 100 mM | 0.0218 mL | 0.1092 mL | 0.2184 mL | 0.5460 mL |
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