10-Shogaol
Based on 5 publication(s) in Google Scholar
[10]-Shogaol is an orally active antioxidant. [10]-Shogaol can be extracted from ginger (Zingiber officinale). [10]-Shogaol1 inhibits COX-2 with an IC50 value of 7.5 μM. [10]-Shogaol inhibits the production of proinflammatory cytokines (IL-1β, IL-6). [10]-Shogaol inhibits NF-κB phosphorylation. [10]-Shogaol has anti-inflammatory effects. [10]-Shogaol has anticancer effects against Docetaxel (HY-B0011)-resistant prostate cancer. [10]-Shogaol exhibits larvicidal activity against L5 larvae of Angiostrongylus cantonensis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.66%
- CAS 番号: 36752-54-2
- 分子式: C21H32O3
- 分子量:332.48
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
![[10]-Shogaol Chemical Structure](https://file.medchemexpress.com/product_pic/hy-n2434.gif "Click to View [10]-Shogaol Chemical Structure")
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
![[8]-Shogaol](https://file.medchemexpress.com/product_pic/hy-n2435.gif)
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![[10]-Shogaol (Standard)](https://file.medchemexpress.com/product_pic/hy-n2434r.gif)
MedChemExpress（MCE）の使用を引用している文献 [10]-Shogaol
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生物活性
製品説明
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COX-2 7.5 μM (IC50) |
IL-1β |
IL-6 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| IMR-32 | ED50 |
3.3 μM
Compound: 12
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Effective dose to protect IMR-32 cells from beta-Amyloid (BA) insult was determined using MTT reduction assay
Effective dose to protect IMR-32 cells from beta-Amyloid (BA) insult was determined using MTT reduction assay
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[PMID: 14980683] |
| PC-12 | ED50 |
1.8 μM
Compound: 12
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Effective dose to protect PC12 cells from beta-Amyloid (BA) insult was determined using MTT reduction assay
Effective dose to protect PC12 cells from beta-Amyloid (BA) insult was determined using MTT reduction assay
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[PMID: 14980683] |
体外実験
[10]-Shogaol (2-10 μM; 12-24 h) promotes the migration and emigration of normal human epidermal keratinocytes and dermal fibroblasts[1].
[10]-Shogaol (2-10 μM) enhances the production of TGF-β, PDGF-αβ, and VEGF in both human normal epidermal keratinocytes and dermal fibroblasts[1].
[10]-Shogaol inhibits COX-2 with an IC50 of 7.5 μM[2].
[10]-Shogaol (100 μM; 24 h) inhibits proliferation of Docetaxel (HY-B0011)-resistant prostate cancer cells (PC3R) and downregulates MRP1 and GSTπ protein expression [3].
[10]-Shogaol (1-200 μM; 24-72 h) shows larvicidal activity against Angiostrongylus cantonensis L5 larvae in a time- and dose-dependent manner[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Human normal epidermal keratinocytes
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Concentration:2 μM, 10 μM
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Incubation Time:12 h, 24 h
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Result:Showed cell migration rates of 204.76% (12 h) and 236.0% (24 h) (at 2 μM).
Showed cell migration rates of 205.65% (12 h) and 250.44% (24 h) (at 10 μM).
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Cell Line:Human dermal fibroblasts
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Concentration:2 μM, 10 μM
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Incubation Time:12 h, 24 h
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Result:Showed cell migration rates of 201.67% (12 h) and 246.06% (24 h) (at 2 μM).
Showed cell migration rates of 187.82% (12 h) and 237.92% (24 h) (at 10 μM).
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male BALB/c mice (5-week-old) with DSS-induced colitis[5]
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Dosage:30 mg/kg
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Administration:Oral administration (p.o.), 2 weeks
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Result:Significantly suppressed clinical symptoms of colitis, including weight loss and increased disease activity index; inhibited the production of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IFN-γ).
Downregulated the expression of iNOS and COX-2.
Inhibited phosphorylation of NF-κB.
Protected intestinal barrier function by increasing mucin expression and maintaining tight junction proteins (ZO-1 and occludin).
化学情報
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CAS 番号 36752-54-2
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性状 Liquid (Density: 0.996±0.06 g/cm3)
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分子量 332.48
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分子式 C21H32O3
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Color Colorless to light yellow
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SMILES
CCCCCCCCC/C=C/C(CCC1=CC=C(O)C(OC)=C1)=O
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Bioact Mater
Lipidomic analysis of plant-derived extracellular vesicles for guidance of potential anti-cancer therapy. [Abstract]2024 Dec 10:46:82-96. PMID: 39737211 -
Food Chem
2024 Sep 15:452:139425. PMID: 38744128 -
Pharm Biol
Potential pharmacological quality control markers in the traditional Japanese medicine Hangeshashinto: identifying anti-inflammatory ingredients through a cell-based bioassay and multicomponent analysis. [Abstract]2025 Dec;63(1):819-836. PMID: 41240012 -
Biosci Biotechnol Biochem
Role of phosphorylation and vanilloid ligand structure in ligand-dependent differential activations of transient receptor potential vanilloid 1. [Abstract]2024 Oct 22;88(11):1316-1325. PMID: 39217101 -
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (300.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.52 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
プロトコル
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
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Pyroptosis Solutions
Pyroptosis is a lytic inflammatory cell-death pathway executed by gasdermin pores, most classically through inflammasome-mediated activation of caspase-1, cleavage of gasdermin D, membrane pore formation, LDH release, and secretion of IL-1β and IL-18. The canonical pathway is commonly modeled by priming cells with an inflammatory signal such as LPS to induce pro-IL-1β and inflammasome components, followed by an activation signal such as ATP or nigericin to activate NLRP3, ASC speck formation, caspase-1 cleavage, GSDMD cleavage, cytokine release, and pyroptotic membrane rupture. The non-canonical pathway is triggered when cytosolic LPS activates mouse caspase-11 or human caspase-4/5, leading to GSDMD cleavage and pyroptosis, and this can secondarily activate NLRP3-dependent IL-1β release. Pyroptosis is linked to inflammatory injury, infection, cancer, liver disease, ocular disease, placental inflammation, and other disease phenotypes, but unresolved questions include which gasdermin fam
純度とドキュメンテーション
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データシート (274 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Chen CY, et al. 10-Shogaol, an antioxidant from Zingiber officinale for skin cell proliferation and migration enhancer. Int J Mol Sci. 2012;13(2):1762-77. [Content Brief]
[2]. van Breemen RB, et al. Cyclooxygenase-2 inhibitors in ginger (Zingiber officinale). Fitoterapia. 2011 Jan;82(1):38-43. [Content Brief]
[3]. Liu CM, et al. Ginger Phytochemicals Inhibit Cell Growth and Modulate Drug Resistance Factors in Docetaxel Resistant Prostate Cancer Cell. Molecules. 2017 Sep 5;22(9). pii: E1477. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0077 mL | 15.0385 mL | 30.0770 mL | 75.1925 mL |
| 5 mM | 0.6015 mL | 3.0077 mL | 6.0154 mL | 15.0385 mL | |
| 10 mM | 0.3008 mL | 1.5038 mL | 3.0077 mL | 7.5192 mL | |
| 15 mM | 0.2005 mL | 1.0026 mL | 2.0051 mL | 5.0128 mL | |
| 20 mM | 0.1504 mL | 0.7519 mL | 1.5038 mL | 3.7596 mL | |
| 25 mM | 0.1203 mL | 0.6015 mL | 1.2031 mL | 3.0077 mL | |
| 30 mM | 0.1003 mL | 0.5013 mL | 1.0026 mL | 2.5064 mL | |
| 40 mM | 0.0752 mL | 0.3760 mL | 0.7519 mL | 1.8798 mL | |
| 50 mM | 0.0602 mL | 0.3008 mL | 0.6015 mL | 1.5038 mL | |
| 60 mM | 0.0501 mL | 0.2506 mL | 0.5013 mL | 1.2532 mL | |
| 80 mM | 0.0376 mL | 0.1880 mL | 0.3760 mL | 0.9399 mL | |
| 100 mM | 0.0301 mL | 0.1504 mL | 0.3008 mL | 0.7519 mL |
[10]-Shogaol 関連分類
HY-N2434 関連分類
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