2. Anti-infection Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis Bacterial
  4. 2,5-Di-tert-butyl-1,4-benzoquinone

2,5-Di-tert-butyl-1,4-benzoquinone is a potent antibacterial agent that defends the emerging antibacterial resistance, which is found in marine Streptomyces sp. VITVSK1. 2,5-Di-tert-butyl-1,4-benzoquinone is also a potent inhibitor of RNA polymerase.

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2,5-Di-tert-butyl-1,4-benzoquinone

2,5-Di-tert-butyl-1,4-benzoquinone Chemical Structure

CAS No. : 2460-77-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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2,5-Di-tert-butyl-1,4-benzoquinone Related Antibodies

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  • Biological Activity

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Description

2,5-Di-tert-butyl-1,4-benzoquinone is a potent antibacterial agent that defends the emerging antibacterial resistance, which is found in marine Streptomyces sp. VITVSK1. 2,5-Di-tert-butyl-1,4-benzoquinone is also a potent inhibitor of RNA polymerase[1][2].

IC50 & Target

RNA Polymerase

 

Cellular Effect
Cell Line Type Value Description References
A2780 IC50
34 μM
Compound: 13
Growth inhibition of human A2780 cells after 72 hrs by SRB assay
Growth inhibition of human A2780 cells after 72 hrs by SRB assay
[PMID: 29519737]
A2780cis IC50
42.5 μM
Compound: 13
Growth inhibition of human A2780cis cells after 72 hrs by SRB assay
Growth inhibition of human A2780cis cells after 72 hrs by SRB assay
[PMID: 29519737]
OVCAR-8 IC50
51.2 μM
Compound: 13
Growth inhibition of human OVCAR8 cells after 72 hrs by SRB assay
Growth inhibition of human OVCAR8 cells after 72 hrs by SRB assay
[PMID: 29519737]
Molecular Weight

220.31

Formula

C14H20O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=C(C(C)(C)C)C(C=C1C(C)(C)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (22.70 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5391 mL 22.6953 mL 45.3906 mL
5 mM 0.9078 mL 4.5391 mL 9.0781 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 0.5 mg/mL (2.27 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.50%

SDS (394 KB)

COA (252 KB) HNMR (114 KB) GC (180 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5391 mL 22.6953 mL 45.3906 mL 113.4765 mL
5 mM 0.9078 mL 4.5391 mL 9.0781 mL 22.6953 mL
10 mM 0.4539 mL 2.2695 mL 4.5391 mL 11.3476 mL
15 mM 0.3026 mL 1.5130 mL 3.0260 mL 7.5651 mL
20 mM 0.2270 mL 1.1348 mL 2.2695 mL 5.6738 mL
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2,5-Di-tert-butyl-1,4-benzoquinone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2,5-Di-tert-butyl-1,4-benzoquinone2460-77-7DNA/RNA SynthesisBacterialantibacterial agentantibacterial resistanceRNA polymeraseInhibitorinhibitorinhibit

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