2-Hydroxy-4-(methylthio)butyric acid
Based on 1 publication(s) in Google Scholar
2-Hydroxy-4-(methylthio)butyric acid (2-Hydroxy-4-(Methylthio)-Butanoic Acid) is an orally active source of methionine. 2-Hydroxy-4-(methylthio)butyric acid reduces the expression of multiple mRNAs (BHMT, MTR, MAT1A, SAHH, and PCK1). 2-Hydroxy-4-(methylthio)butyric acid increases milk production in periparturient cows.
For research use only. We do not sell to patients.
- Purity: 88.0%
- CAS No.: 583-91-5
- Formula: C5H10O3S
- Molecular Weight:150.20
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 2-Hydroxy-4-(methylthio)butyric acid
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Biological Activity
2-Hydroxy-4-(methylthio)butyric acid (10-60 μM; 24 h) linearly decreases the mRNA expression of BHMT, MTR, MAT1A, and PCK1, linearly decreases SAHH and G6PC mRNA expression, and has no significant effect on CPT1a mRNA expression in primary hepatocytes isolated from Holstein calves[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
2-Hydroxy-4-(methylthio)butyric acid (prepartum: 0.09%, 0.18% of diet DM; postpartum: 0.13%, 0.20% of diet DM; p.o. via diet; from 21 d prepartum to 84 d postpartum) increases milk yield quadratically, with the highest yield in the 0.09% (prepartum) and 0.13% (postpartum) groups, but does not improve hepatic lipid metabolism in periparturient Holstein cows[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 583-91-5
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Appearance Liquid (Density: 1.22 g/cm³)
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Molecular Weight 150.20
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Formula C5H10O3S
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Color Light yellow to brown
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SMILES
O=C(C(CCSC)O)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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iScience
Comparative analysis reveals molecular adaptation of mammalian HCA2 to microbial metabolites. [Abstract]2026 May 22;29(6):116030. PMID: 42221821
Solvent & Solubility
DMSO : ≥ 200 mg/mL (1331.56 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (33.29 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (33.29 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Fang Z, et al. Methionine metabolism in piglets Fed DL-methionine or its hydroxy analogue was affected by distribution of enzymes oxidizing these sources to keto-methionine. J Agric Food Chem. 2010 Feb 10;58(3):2008-14. [Content Brief]
[2]. St-Pierre NR, et al. Effects of 2-hydroxy-4-(methylthio) butanoic acid (HMB) and its isopropyl ester on milk production and composition by Holstein cows. J Dairy Sci. 2005 Jul;88(7):2487-97. [Content Brief]
[3]. Noftsger SM, et al. Effects of 2-hydroxy-4-(methylthio) butanoic acid (HMB) on microbial growth in continuous culture. J Dairy Sci. 2003 Aug;86(8):2629-36. [Content Brief]
[4]. Piepenbrink MS, et al. Feeding 2-hydroxy-4-(methylthio)-butanoic acid to periparturient dairy cows improves milk production but not hepatic metabolism. J Dairy Sci. 2004 Apr;87(4):1071-84. [Content Brief]
[5]. Zhang Q, et al. The effect of increasing concentrations of dl-methionine and 2-hydroxy-4-(methylthio) butanoic acid on hepatic genes controlling methionine regeneration and gluconeogenesis. J Dairy Sci. 2016 Oct;99(10):8451-8460. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.6578 mL | 33.2889 mL | 66.5779 mL | 166.4447 mL |
| 5 mM | 1.3316 mL | 6.6578 mL | 13.3156 mL | 33.2889 mL | |
| 10 mM | 0.6658 mL | 3.3289 mL | 6.6578 mL | 16.6445 mL | |
| 15 mM | 0.4439 mL | 2.2193 mL | 4.4385 mL | 11.0963 mL | |
| 20 mM | 0.3329 mL | 1.6644 mL | 3.3289 mL | 8.3222 mL | |
| 25 mM | 0.2663 mL | 1.3316 mL | 2.6631 mL | 6.6578 mL | |
| 30 mM | 0.2219 mL | 1.1096 mL | 2.2193 mL | 5.5482 mL | |
| 40 mM | 0.1664 mL | 0.8322 mL | 1.6644 mL | 4.1611 mL | |
| 50 mM | 0.1332 mL | 0.6658 mL | 1.3316 mL | 3.3289 mL | |
| 60 mM | 0.1110 mL | 0.5548 mL | 1.1096 mL | 2.7741 mL | |
| 80 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0806 mL | |
| 100 mM | 0.0666 mL | 0.3329 mL | 0.6658 mL | 1.6644 mL |
2-Hydroxy-4-(methylthio)butyric acid Related Classifications
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