2'-O,4'-C-Methyleneguanosine
Based on 1 Customer Validation
2′-O,4′-C-Methyleneguanosine (LNA-G) is a reverse guanine analog, where LNA (locked nucleic acid) is a nucleic acid analog. LNA modification can be widely used in various fields, such as effective binding affinity with complementary sequences and stronger nuclease resistance than natural nucleotides, providing great potential for application in disease diagnosis and research. 2'-O,4'-C-Methyleneguanosine is a substrate for KOD DNA polymerase, which incorporates LNA-G nucleotides into growing DNA strands, including consecutive incorporations , to generate full-length extension products.
For research use only. We do not sell to patients.
- Purity: 99.22%
- CAS No.: 207131-16-6
- Formula: C11H13N5O5
- Molecular Weight:295.25
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
|
DNA Polymerase |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | EC50 |
>50 μM
Compound: 7
|
Antiviral activity against wild type Hepatitis C virus genotype 1b infected in human HuH7 cells after 72 hrs by luciferase reporter gene assay
Antiviral activity against wild type Hepatitis C virus genotype 1b infected in human HuH7 cells after 72 hrs by luciferase reporter gene assay
|
[PMID: 24815510] |
2'-O,4'-C-Methyleneguanosine as part of an all-LNA nucleotide mix is efficiently incorporated successively by KOD DNA polymerase to produce a full-length 40-nucleotide LNA-modified DNA strand that is highly resistant to 3'→5' exonuclease degradation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 207131-16-6
-
Appearance Solid
-
Molecular Weight 295.25
-
Formula C11H13N5O5
-
Color White to off-white
-
SMILES
OC[C@]12[C@@H](O)[C@@H](OC2)[C@H](N3C(N=C(N)NC4=O)=C4N=C3)O1
-
Synonyms
LNA-G
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 35 mg/mL (118.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3870 mL | 16.9348 mL | 33.8696 mL | 84.6740 mL |
| 5 mM | 0.6774 mL | 3.3870 mL | 6.7739 mL | 16.9348 mL | |
| 10 mM | 0.3387 mL | 1.6935 mL | 3.3870 mL | 8.4674 mL | |
| 15 mM | 0.2258 mL | 1.1290 mL | 2.2580 mL | 5.6449 mL | |
| 20 mM | 0.1693 mL | 0.8467 mL | 1.6935 mL | 4.2337 mL | |
| 25 mM | 0.1355 mL | 0.6774 mL | 1.3548 mL | 3.3870 mL | |
| 30 mM | 0.1129 mL | 0.5645 mL | 1.1290 mL | 2.8225 mL | |
| 40 mM | 0.0847 mL | 0.4234 mL | 0.8467 mL | 2.1169 mL | |
| 50 mM | 0.0677 mL | 0.3387 mL | 0.6774 mL | 1.6935 mL | |
| 60 mM | 0.0564 mL | 0.2822 mL | 0.5645 mL | 1.4112 mL | |
| 80 mM | 0.0423 mL | 0.2117 mL | 0.4234 mL | 1.0584 mL | |
| 100 mM | 0.0339 mL | 0.1693 mL | 0.3387 mL | 0.8467 mL |
2'-O,4'-C-Methyleneguanosine Related Classifications
HY-W406070 Related Classifications
Powered by Bioz