2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. 3-Indoxyl phosphate sodium

3-Indoxyl phosphate sodium  (Synonyms: 3-IP sodium)

Cat. No.: HY-W009458
Handling Instructions Technical Support

3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.

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3-Indoxyl phosphate sodium

3-Indoxyl phosphate sodium Chemical Structure

CAS No. : 3318-43-2

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Description

3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.

Molecular Weight

257.09

Formula

C8H6NNa2O4P
CAS No.
SMILES

O=P(OC1=CNC2=C1C=CC=C2)(O[Na])O[Na]
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : 33.33 mg/mL (129.64 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8897 mL 19.4484 mL 38.8969 mL
5 mM 0.7779 mL 3.8897 mL 7.7794 mL
View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.8897 mL 19.4484 mL 38.8969 mL 97.2422 mL
5 mM 0.7779 mL 3.8897 mL 7.7794 mL 19.4484 mL
10 mM 0.3890 mL 1.9448 mL 3.8897 mL 9.7242 mL
15 mM 0.2593 mL 1.2966 mL 2.5931 mL 6.4828 mL
20 mM 0.1945 mL 0.9724 mL 1.9448 mL 4.8621 mL
25 mM 0.1556 mL 0.7779 mL 1.5559 mL 3.8897 mL
30 mM 0.1297 mL 0.6483 mL 1.2966 mL 3.2414 mL
40 mM 0.0972 mL 0.4862 mL 0.9724 mL 2.4311 mL
50 mM 0.0778 mL 0.3890 mL 0.7779 mL 1.9448 mL
60 mM 0.0648 mL 0.3241 mL 0.6483 mL 1.6207 mL
80 mM 0.0486 mL 0.2431 mL 0.4862 mL 1.2155 mL
100 mM 0.0389 mL 0.1945 mL 0.3890 mL 0.9724 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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3-Indoxyl phosphate sodium Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Indoxyl phosphate3318-43-23-IPPhosphataseInhibitorinhibitorinhibit

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