3-Indoxyl phosphate sodium
3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.
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- CAS No.: 3318-43-2
- Formula: C8H6NNa2O4P
- Molecular Weight:257.09
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 3318-43-2
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Molecular Weight 257.09
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Formula C8H6NNa2O4P
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SMILES
O=P(OC1=CNC2=C1C=CC=C2)(O[Na])O[Na]
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Synonyms
3-IP sodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O : 33.33 mg/mL (129.64 mM; Need ultrasonic)
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.8897 mL | 19.4484 mL | 38.8969 mL | 97.2422 mL |
| 5 mM | 0.7779 mL | 3.8897 mL | 7.7794 mL | 19.4484 mL | |
| 10 mM | 0.3890 mL | 1.9448 mL | 3.8897 mL | 9.7242 mL | |
| 15 mM | 0.2593 mL | 1.2966 mL | 2.5931 mL | 6.4828 mL | |
| 20 mM | 0.1945 mL | 0.9724 mL | 1.9448 mL | 4.8621 mL | |
| 25 mM | 0.1556 mL | 0.7779 mL | 1.5559 mL | 3.8897 mL | |
| 30 mM | 0.1297 mL | 0.6483 mL | 1.2966 mL | 3.2414 mL | |
| 40 mM | 0.0972 mL | 0.4862 mL | 0.9724 mL | 2.4311 mL | |
| 50 mM | 0.0778 mL | 0.3890 mL | 0.7779 mL | 1.9448 mL | |
| 60 mM | 0.0648 mL | 0.3241 mL | 0.6483 mL | 1.6207 mL | |
| 80 mM | 0.0486 mL | 0.2431 mL | 0.4862 mL | 1.2155 mL | |
| 100 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9724 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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