3318-43-2
Chemical Structure
3-Indoxyl phosphate sodium
Synonym(s): 3-IP sodium
- CAS No.: 3318-43-2
- Formula:C8H6NNa2O4P
- Molecular Weight:257.09
IUPAC Name: Sodium 1H-indol-3-yl phosphate
InChIKey: WCSKWBKPKXJWEG-UHFFFAOYSA-L
SMILES: O=P(OC1=CNC2=C1C=CC=C2)(O[Na])O[Na]
Biological Activity: 3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.
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3-Indoxyl phosphate sodium | 3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs. | |||||||||||||||||||||
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