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  4. 7-Ethoxy-4-trifluoromethylcoumarin

7-Ethoxy-4-trifluoromethylcoumarin is a fluorescent substrate of various enzymes of the cytochrome P450 superfamily

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7-Ethoxy-4-trifluoromethylcoumarin

7-Ethoxy-4-trifluoromethylcoumarin Chemical Structure

CAS No. : 115453-82-2

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Based on 1 publication(s) in Google Scholar

7-Ethoxy-4-trifluoromethylcoumarin Related Antibodies

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Description

7-Ethoxy-4-trifluoromethylcoumarin is a fluorescent substrate of various enzymes of the cytochrome P450 superfamily[1]

Molecular Weight

258.19

Formula

C12H9F3O3
CAS No.
Appearance

Solid

Color

Off-white to pink

Emission (Em)

454
Excitation (Ex)

344
SMILES

O=C1C=C(C(F)(F)F)C2=CC=C(OCC)C=C2O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (193.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8731 mL 19.3656 mL 38.7312 mL
5 mM 0.7746 mL 3.8731 mL 7.7462 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8731 mL 19.3656 mL 38.7312 mL 96.8279 mL
5 mM 0.7746 mL 3.8731 mL 7.7462 mL 19.3656 mL
10 mM 0.3873 mL 1.9366 mL 3.8731 mL 9.6828 mL
15 mM 0.2582 mL 1.2910 mL 2.5821 mL 6.4552 mL
20 mM 0.1937 mL 0.9683 mL 1.9366 mL 4.8414 mL
25 mM 0.1549 mL 0.7746 mL 1.5492 mL 3.8731 mL
30 mM 0.1291 mL 0.6455 mL 1.2910 mL 3.2276 mL
40 mM 0.0968 mL 0.4841 mL 0.9683 mL 2.4207 mL
50 mM 0.0775 mL 0.3873 mL 0.7746 mL 1.9366 mL
60 mM 0.0646 mL 0.3228 mL 0.6455 mL 1.6138 mL
80 mM 0.0484 mL 0.2421 mL 0.4841 mL 1.2103 mL
100 mM 0.0387 mL 0.1937 mL 0.3873 mL 0.9683 mL
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7-Ethoxy-4-trifluoromethylcoumarin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Ethoxy-4-trifluoromethylcoumarin115453-82-2Fluorescent DyeCytochromeP450Inhibitorinhibitorinhibit

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