3'-O-(2-Nitrobenzyl)-dATP 

3'-O-(2-Nitrobenzyl)-dATP is a reversible and photolabile DNA synthesis terminator. 3'-O-(2-Nitrobenzyl)-dATP incorporates into growing DNA strands to terminate synthesis in a base-specific manner. 3'-O-(2-Nitrobenzyl)-dATP undergoes efficient photolytic removal of its 3'-protecting group upon UV exposure to enable reinitiation of DNA synthesis. 3'-O-(2-Nitrobenzyl)-dATP supports development of the Base Addition Sequencing Scheme (BASS) via a complete stop-start DNA synthesis cycle^[1].

3'-O-(2-Nitrobenzyl)-dATP (0.25-1 mM; 10 min) terminates Bst DNA polymerase-mediated DNA synthesis^[1].
3'-O-(2-Nitrobenzyl)-dATP (250 μM; 10 min) terminates Bst DNA polymerase-mediated DNA synthesis in a base-specific manner; the 2-nitrobenzyl protecting group is efficiently removed by 10 minutes of UV exposure, allowing reinitiation of DNA synthesis^[1].
3'-O-(2-Nitrobenzyl)-dATP (250 μM) induces incomplete termination of AmpliTaq® and VentR® exo- DNA polymerase-mediated DNA synthesis, induces complete termination of Bst DNA polymerase-mediated DNA synthesis, and inhibits Sequenase®-mediated DNA synthesis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

626.30

C₁₇H₂₁N₆O₁₄P₃

159954-97-9

NC1=NC=NC2=C1N=CN2[C@H]3C[C@H](OCC4=CC=CC=C4[N+]([O-])=O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Metzker ML, et al. Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates. Nucleic Acids Res. 1994;22(20):4259-4267.  [Content Brief]