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Cat. No.: HY-177901

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3'-O-(2-Nitrobenzyl)-dATP

3'-O-(2-Nitrobenzyl)-dATP is a reversible and photolabile DNA synthesis terminator. 3'-O-(2-Nitrobenzyl)-dATP incorporates into growing DNA strands to terminate synthesis in a base-specific manner. 3'-O-(2-Nitrobenzyl)-dATP undergoes efficient photolytic removal of its 3'-protecting group upon UV exposure to enable reinitiation of DNA synthesis. 3'-O-(2-Nitrobenzyl)-dATP supports development of the Base Addition Sequencing Scheme (BASS) via a complete stop-start DNA synthesis cycle.

For research use only. We do not sell to patients.

CAS No.: 159954-97-9
Formula: C₁₇H₂₁N₆O₁₄P₃
Molecular Weight:626.30

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

3'-O-(2-Nitrobenzyl)-dATP Chemical Structure

CAS No.: 159954-97-9

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Biological Activity

Description

All DNA/RNA Synthesis Isoforms :

In Vitro

3'-O-(2-Nitrobenzyl)-dATP (0.25-1 mM; 10 min) terminates Bst DNA polymerase-mediated DNA synthesis^[1].
3'-O-(2-Nitrobenzyl)-dATP (250 μM; 10 min) terminates Bst DNA polymerase-mediated DNA synthesis in a base-specific manner; the 2-nitrobenzyl protecting group is efficiently removed by 10 minutes of UV exposure, allowing reinitiation of DNA synthesis^[1].
3'-O-(2-Nitrobenzyl)-dATP (250 μM) induces incomplete termination of AmpliTaq® and VentR® exo- DNA polymerase-mediated DNA synthesis, induces complete termination of Bst DNA polymerase-mediated DNA synthesis, and inhibits Sequenase®-mediated DNA synthesis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 159954-97-9
Molecular Weight 626.30
Formula C₁₇H₂₁N₆O₁₄P₃
SMILES

NC1=NC=NC2=C1N=CN2[C@H]3C[C@H](OCC4=CC=CC=C4[N+]([O-])=O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O3
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Metzker ML, et al. Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates. Nucleic Acids Res. 1994;22(20):4259-4267. [Content Brief]

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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3'-O-(2-Nitrobenzyl)-dATP

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

3'-O-(2-Nitrobenzyl)-dATP Related Classifications

HY-177901 Related Classifications