(3R,4R)-A2-32-01
Based on 2 publication(s) in Google Scholar
(3R,4R)-A2-32-01 (compound 24(R,R)), the (R,R)-enantiomer of A2-32-01, is a Staphylococcus aureus caseinolytic protease (SaClpP) inhibitor.
For research use only. We do not sell to patients.
- Purity: 98.47%
- CAS No.: 1359752-95-6
- Formula: C19H27NO2
- Molecular Weight:301.42
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) (3R,4R)-A2-32-01
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HaCaT | EC50 |
75 μM
Compound: 2
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Cytotoxicity against human HaCaT cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 21855356] |
| NIH3T3 | EC50 |
50 μM
Compound: 2
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Cytotoxicity against mouse NIH/3T3 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against mouse NIH/3T3 cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 21855356] |
(3R,4R)-A2-32-01 is a β-lactone compound that covalently reacts with the catalytic site of ClpP to form a β-hydroxyacyl-enzyme complex, thereby inhibiting the proteolysis reaction[1].
(3R,4R)-A2-32-01 exhibits a relatively weaker inhibitory activity against SaClpP compared with the S,S configuration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1359752-95-6
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Appearance Liquid (Density: 1.010±0.06 g/cm3)
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Molecular Weight 301.42
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Formula C19H27NO2
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Color Colorless to light yellow
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SMILES
O=C1O[C@H](CCC2=CC=CN=C2)[C@H]1CCCCCCCC=C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Autophagy
2022 Jun;18(6):1416-1432. PMID: 34720024 -
Nat Commun
Utilizing nanoinducers for precision degradation of bacterial protein to mitigate antibiotic resistance. [Abstract]2025 Dec 18;16(1):11204. PMID: 41413196
Solvent & Solubility
DMSO : 100 mg/mL (331.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3176 mL | 16.5881 mL | 33.1763 mL | 82.9407 mL |
| 5 mM | 0.6635 mL | 3.3176 mL | 6.6353 mL | 16.5881 mL | |
| 10 mM | 0.3318 mL | 1.6588 mL | 3.3176 mL | 8.2941 mL | |
| 15 mM | 0.2212 mL | 1.1059 mL | 2.2118 mL | 5.5294 mL | |
| 20 mM | 0.1659 mL | 0.8294 mL | 1.6588 mL | 4.1470 mL | |
| 25 mM | 0.1327 mL | 0.6635 mL | 1.3271 mL | 3.3176 mL | |
| 30 mM | 0.1106 mL | 0.5529 mL | 1.1059 mL | 2.7647 mL | |
| 40 mM | 0.0829 mL | 0.4147 mL | 0.8294 mL | 2.0735 mL | |
| 50 mM | 0.0664 mL | 0.3318 mL | 0.6635 mL | 1.6588 mL | |
| 60 mM | 0.0553 mL | 0.2765 mL | 0.5529 mL | 1.3823 mL | |
| 80 mM | 0.0415 mL | 0.2074 mL | 0.4147 mL | 1.0368 mL | |
| 100 mM | 0.0332 mL | 0.1659 mL | 0.3318 mL | 0.8294 mL |
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