4-Chloro-3-sulfamoylbenzoic acid
Based on 1 Customer Validation
4-Chloro-3-sulfamoylbenzoic acid is a weak inhibitor of human carbonic anhydrase isoforms hCA I, hCA II, hCA IV, and hCA IX, and a synthesis intermediate for carbonic anhydrase inhibitors. 4-Chloro-3-sulfamoylbenzoic acid is the major metabolite of tripamide detected in tissues, urine, and feces of rats and rabbits following Tripamide (HY-106570) administration. 4-Chloro-3-sulfamoylbenzoic acid can be used for the study of carbonic anhydrase inhibition and species differences in drug metabolism.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 1205-30-7
- Formula: C7H6ClNO4S
- Molecular Weight:235.64
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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hCA I 8847 nM (Ki) |
hCA II 1382 nM (Ki) |
hCA IX 8976 nM (Ki) |
4-Chloro-3-sulfamoylbenzoic acid (3b) (0.01 nM-10 μM; 10 min preincubation) inhibits recombinant human hCA I (Ki = 5750 nM), hCA II (Ki = 760.0 nM), hCA IV (Ki = 957.5 nM), and hCA IX (Ki = 9570 nM) in a cell-free biochemical assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
14C-4-Chloro-3-sulfamoylbenzoic acid (0.24 mg/kg; i.v.; single dose) has biphasic blood half-lives of 1.7 and 49.3 minutes in male New Zealand White rabbits, with nearly complete urinary excretion (100% of dose) within 8 hours[2].
4-Chloro-3-sulfamoylbenzoic acid (p.o.; single dose) is the dominant metabolite after a single oral administration of Tripamide (HY-106570) in male Wistar rats and male New Zealand White rabbits[2].
4-Chloro-3-sulfamoylbenzoic acid (p.o.; single dose; 24 hours) is excreted 10.1% in urine and 5.6% in feces in male Wistar rats, and at 40.2% of the dose in urine and 0.5% in feces in male New Zealand White rabbits over 24 hours after oral [14C]tripamide administration[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1205-30-7
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Appearance Solid
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Molecular Weight 235.64
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Formula C7H6ClNO4S
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Color White to off-white
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SMILES
ClC1=C(S(N)(=O)=O)C=C(C(O)=O)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 50 mg/mL (212.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Swain B, et al. Synthesis of a new series of 3-functionalised-1-phenyl-1,2,3-triazole sulfamoylbenzamides as carbonic anhydrase I, II, IV and IX inhibitors. J Enzyme Inhib Med Chem. 2019;34(1):1199-1209. [Content Brief]
[2]. Horie T, et al. Studies on metabolism of tripamide. III. Metabolic fate of 14C-tripamide in rats and rabbits. Jpn J Pharmacol. 1982;32(6):1041-1049. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.2438 mL | 21.2188 mL | 42.4376 mL | 106.0940 mL |
| 5 mM | 0.8488 mL | 4.2438 mL | 8.4875 mL | 21.2188 mL | |
| 10 mM | 0.4244 mL | 2.1219 mL | 4.2438 mL | 10.6094 mL | |
| 15 mM | 0.2829 mL | 1.4146 mL | 2.8292 mL | 7.0729 mL | |
| 20 mM | 0.2122 mL | 1.0609 mL | 2.1219 mL | 5.3047 mL | |
| 25 mM | 0.1698 mL | 0.8488 mL | 1.6975 mL | 4.2438 mL | |
| 30 mM | 0.1415 mL | 0.7073 mL | 1.4146 mL | 3.5365 mL | |
| 40 mM | 0.1061 mL | 0.5305 mL | 1.0609 mL | 2.6524 mL | |
| 50 mM | 0.0849 mL | 0.4244 mL | 0.8488 mL | 2.1219 mL | |
| 60 mM | 0.0707 mL | 0.3536 mL | 0.7073 mL | 1.7682 mL | |
| 80 mM | 0.0530 mL | 0.2652 mL | 0.5305 mL | 1.3262 mL | |
| 100 mM | 0.0424 mL | 0.2122 mL | 0.4244 mL | 1.0609 mL |
4-Chloro-3-sulfamoylbenzoic acid Related Classifications
HY-W012168 Related Classifications
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