TRITC
Based on 1 publication(s) in Google Scholar
TRITC (5(6)-Tetramethylrhodamine isothiocyanate) is a commonly used fluorescent dye, belonging to the Rhodamine derivative family. It exhibits red fluorescence properties (Ex/Em ≈ 550/580 nm). TRITC can be used for cell labeling and imaging.
For research use only. We do not sell to patients.
- Purity: 96.38%
- CAS No.: 95197-95-8
- Formula: C25H21N3O3S
- Molecular Weight:443.52
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) TRITC
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Biological Activity
TRITC (0.3 μg/L; 5 h) contained in RHCDs is rapidly taken up by A549 cells, accumulates exclusively in the cytoplasm, and shows excellent biocompatibility with A549 cells[1].
TRITC, when encapsulated in PLGA nanoparticles, traverses the cell membrane to localize in the cytoplasm of ZP-free GV-stage mouse oocytes after 4 h of incubation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 human lung adenocarcinoma cells
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Concentration:0.3 μg/L (as part of 40 μg/L RHCDs)
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Incubation Time:5 h
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Result:Localized red fluorescence to the cytoplasm of A549 cells, with no fluorescence detected in the cell nucleus.
Showed no obvious cytotoxicity to A549 cells, with cell survival rates above 90%.
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Chemical Information
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CAS No. 95197-95-8
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Appearance Solid
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Molecular Weight 443.52
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Formula C25H21N3O3S
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Color Brown to dark brown
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SMILES
CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(N=C=S)C=C4C([O-])=O)=C2C=C1)C.CN(C5=CC6=[O+]C7=C(C=CC(N(C)C)=C7)C(C8=CC=CC(N=C=S)=C8C([O-])=O)=C6C=C5)C
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Synonyms
5(6)-Tetramethylrhodamine isothiocyanate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 10 mg/mL (22.55 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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Handling Instructions (2659 KB)
References
[1]. Tang M, et al. Hollow carbon dots labeled with FITC or TRITC for use in fluorescent cellular imaging. Mikrochim Acta. 2018;185(4):223. Published 2018 Mar 14. [Content Brief]
[2]. Kim HJ, et al. TRITC-Loaded PLGA Nanoparticles as Drug Delivery Carriers in Mouse Oocytes and Embryos. ACS Appl Mater Interfaces. 2021;13(5):5975-5988. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2547 mL | 11.2734 mL | 22.5469 mL | 56.3672 mL |
| 5 mM | 0.4509 mL | 2.2547 mL | 4.5094 mL | 11.2734 mL | |
| 10 mM | 0.2255 mL | 1.1273 mL | 2.2547 mL | 5.6367 mL | |
| 15 mM | 0.1503 mL | 0.7516 mL | 1.5031 mL | 3.7578 mL | |
| 20 mM | 0.1127 mL | 0.5637 mL | 1.1273 mL | 2.8184 mL |
TRITC Related Classifications
HY-D0791 Related Classifications
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