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  4. TRITC

TRITC  (Synonyms: 5(6)-Tetramethylrhodamine isothiocyanate)

Cat. No.: HY-D0791 Purity: 96.38%
COA
SDS
Handling Instructions Technical Support

TRITC (5(6)-Tetramethylrhodamine isothiocyanate) is a commonly used fluorescent dye, belonging to the Rhodamine derivative family. It exhibits red fluorescence properties (Ex/Em ≈ 550/580 nm). TRITC can be used for cell labeling and imaging.

For research use only. We do not sell to patients.

TRITC

TRITC Chemical Structure

CAS No. : 95197-95-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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5 mg In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

TRITC Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE TRITC

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

TRITC (5(6)-Tetramethylrhodamine isothiocyanate) is a commonly used fluorescent dye, belonging to the Rhodamine derivative family. It exhibits red fluorescence properties (Ex/Em ≈ 550/580 nm). TRITC can be used for cell labeling and imaging[1][2].

In Vitro

TRITC (0.3 μg/L; 5 h) contained in RHCDs is rapidly taken up by A549 cells, accumulates exclusively in the cytoplasm, and shows excellent biocompatibility with A549 cells[1].
TRITC, when encapsulated in PLGA nanoparticles, traverses the cell membrane to localize in the cytoplasm of ZP-free GV-stage mouse oocytes after 4 h of incubation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TRITC Related Antibodies

Immunofluorescence[1]

Cell Line: A549 human lung adenocarcinoma cells
Concentration: 0.3 μg/L (as part of 40 μg/L RHCDs)
Incubation Time: 5 h
Result: Localized red fluorescence to the cytoplasm of A549 cells, with no fluorescence detected in the cell nucleus.
Showed no obvious cytotoxicity to A549 cells, with cell survival rates above 90%.
Molecular Weight

443.52

Formula

C25H21N3O3S
CAS No.
Appearance

Solid

Color

Brown to dark brown

Emission (Em)

570
Excitation (Ex)

544
SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(N=C=S)C=C4C([O-])=O)=C2C=C1)C.CN(C5=CC6=[O+]C7=C(C=CC(N(C)C)=C7)C(C8=CC=CC(N=C=S)=C8C([O-])=O)=C6C=C5)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (22.55 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2547 mL 11.2734 mL 22.5469 mL
5 mM 0.4509 mL 2.2547 mL 4.5094 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.38%

SDS (558 KB)

COA (250 KB) HNMR (300 KB) LCMS (99 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2547 mL 11.2734 mL 22.5469 mL 56.3672 mL
5 mM 0.4509 mL 2.2547 mL 4.5094 mL 11.2734 mL
10 mM 0.2255 mL 1.1273 mL 2.2547 mL 5.6367 mL
15 mM 0.1503 mL 0.7516 mL 1.5031 mL 3.7578 mL
20 mM 0.1127 mL 0.5637 mL 1.1273 mL 2.8184 mL
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TRITC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TRITC95197-95-85(6)-Tetramethylrhodamine isothiocyanateFluorescent Dyecancer cellszona pellucidaMII-stage mouse oocytescytoplasmhollow carbon dotsA549 cellsGV-stage mouse oocytesmouse oocytesblastocyst stagePLGA nanoparticlesInhibitorinhibitorinhibit

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