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  4. Rhodamine B hydrazide

Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO).
Excitation/emission wavelength:
Conventional detection: 510/578 nm.
Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product).

For research use only. We do not sell to patients.

CAS No. : 74317-53-6

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Based on 1 publication(s) in Google Scholar

Rhodamine B hydrazide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Rhodamine B hydrazide

Cell Imaging/Staining

    Rhodamine B hydrazide purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Sep 15.

    CLSM images of copper ion uptake in MC38 cells after different treatments. Copper ions were stained with Rhodamine B hydrazide (RBH; 10 μM; 30 min).

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    • Purity & Documentation

    • References

    • Customer Review

    Description

    Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO)[1][2][3].
    Excitation/emission wavelength:
    Conventional detection: 510/578 nm.
    Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product).
    In Vitro

    Rhodamine B hydrazide is a colorless and non-fluorescent rhodamine derivative. While the emission is related to the concentration of sulfite (5-800 ng/mL; detection limit=1.4 ng/mL (3σ)). Sulfite reduces dissolved oxygen to yield superoxide radicals, which binds to Rhodamine B hydrazide to form Rhodamine B. Rhodamine B hydrazide gives Rhodamine B-like fluorescence in the presence of sulfite, which is enhanced by Tween 80 surfactant micelles. Rhodamine B hydrazide has an absorption maximum at 554 nm and a fluorescence emission maximum at 574 nm[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Rhodamine B hydrazide Related Antibodies
    Molecular Weight

    456.58

    Formula

    C28H32N4O2
    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    CCN(C1=CC2=C(C3(C4=CC=C(C=C4O2)N(CC)CC)C5=C(C(N3N)=O)C=CC=C5)C=C1)CC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (219.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1902 mL 10.9510 mL 21.9020 mL
    5 mM 0.4380 mL 2.1902 mL 4.3804 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
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    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.87%

    SDS (393 KB)

    COA (257 KB) HNMR (133 KB) RP-HPLC (226 KB) LCMS (97 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1902 mL 10.9510 mL 21.9020 mL 54.7549 mL
    5 mM 0.4380 mL 2.1902 mL 4.3804 mL 10.9510 mL
    10 mM 0.2190 mL 1.0951 mL 2.1902 mL 5.4755 mL
    15 mM 0.1460 mL 0.7301 mL 1.4601 mL 3.6503 mL
    20 mM 0.1095 mL 0.5475 mL 1.0951 mL 2.7377 mL
    25 mM 0.0876 mL 0.4380 mL 0.8761 mL 2.1902 mL
    30 mM 0.0730 mL 0.3650 mL 0.7301 mL 1.8252 mL
    40 mM 0.0548 mL 0.2738 mL 0.5475 mL 1.3689 mL
    50 mM 0.0438 mL 0.2190 mL 0.4380 mL 1.0951 mL
    60 mM 0.0365 mL 0.1825 mL 0.3650 mL 0.9126 mL
    80 mM 0.0274 mL 0.1369 mL 0.2738 mL 0.6844 mL
    100 mM 0.0219 mL 0.1095 mL 0.2190 mL 0.5475 mL
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    Rhodamine B hydrazide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Rhodamine B hydrazide74317-53-6Fluorescent DyeInhibitorinhibitorinhibit

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