Acetaminophen metabolite 3-hydroxy-acetaminophen
Based on 1 Customer Validation
Acetaminophen metabolite 3-hydroxy-acetaminophen (3-Hydroxyacetaminophen) is a non-toxic metabolite and antioxidant of acetaminophen (HY-66005) with free radical scavenging activity. Acetaminophen metabolite 3-hydroxy-acetaminophen can reduce oxidative damage by exerting electron donation ability and antioxidant activity through phenolic hydroxyl groups. 3-hydroxy-acetaminophen can be used to study the toxicity mechanism and drug metabolism of acetaminophen.
For research use only. We do not sell to patients.
- Purity: 99.06%
- CAS No.: 37519-14-5
- Formula: C8H9NO3
- Molecular Weight:167.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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Antioxidant Compound Library
HY-L037
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Drug Metabolite Library
HY-L098
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Radioprotector Library
HY-L178
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Anti-Fibrosis Compound Library
HY-L185
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
3-hydroxy-acetaminophen scavenges free radicals through the electron donation ability of the phenolic hydroxyl group, showing antioxidant activity and can reduce cellular oxidative damage[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 37519-14-5
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Appearance Solid
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Molecular Weight 167.16
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Formula C8H9NO3
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Color White to gray
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SMILES
OC1=C(O)C=C(NC(C)=O)C=C1
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Synonyms
3-Hydroxyacetaminophen
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 250 mg/mL (1495.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.33 mg/mL (13.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (23.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.33 mg/mL (13.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (23.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.9823 mL | 29.9115 mL | 59.8229 mL | 149.5573 mL |
| 5 mM | 1.1965 mL | 5.9823 mL | 11.9646 mL | 29.9115 mL | |
| 10 mM | 0.5982 mL | 2.9911 mL | 5.9823 mL | 14.9557 mL | |
| 15 mM | 0.3988 mL | 1.9941 mL | 3.9882 mL | 9.9705 mL | |
| 20 mM | 0.2991 mL | 1.4956 mL | 2.9911 mL | 7.4779 mL | |
| 25 mM | 0.2393 mL | 1.1965 mL | 2.3929 mL | 5.9823 mL | |
| 30 mM | 0.1994 mL | 0.9970 mL | 1.9941 mL | 4.9852 mL | |
| 40 mM | 0.1496 mL | 0.7478 mL | 1.4956 mL | 3.7389 mL | |
| 50 mM | 0.1196 mL | 0.5982 mL | 1.1965 mL | 2.9911 mL | |
| 60 mM | 0.0997 mL | 0.4985 mL | 0.9970 mL | 2.4926 mL | |
| 80 mM | 0.0748 mL | 0.3739 mL | 0.7478 mL | 1.8695 mL | |
| 100 mM | 0.0598 mL | 0.2991 mL | 0.5982 mL | 1.4956 mL |
Acetaminophen metabolite 3-hydroxy-acetaminophen Related Classifications
HY-G0004 Related Classifications
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