1. Apoptosis
  2. Caspase
  3. BOC-D-FMK

BOC-D-FMK 

Cat. No.: HY-13229 Purity: >95.0%
Handling Instructions

Boc-D-FMK is a cell-permeable, irreversible and broad spectrum caspase inhibitor; inhibits apoptosis stimulated by TNF-α with an IC50 of 39 µM.

For research use only. We do not sell to patients.

BOC-D-FMK Chemical Structure

BOC-D-FMK Chemical Structure

CAS No. : 634911-80-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 174 In-stock
Estimated Time of Arrival: December 31
1 mg USD 60 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 In-stock
Estimated Time of Arrival: December 31
10 mg USD 270 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1030 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

    BOC-D-FMK purchased from MCE. Usage Cited in: Sci Rep. 2017 Jun 7;7(1):2929.

    Caspase signaling pathway. MCF7 and MDA-MB-231 cells are pretreated with 10 µM Z-VAD-FMK and BOC-D-FMK for 1 h and then exposed to 80 μM ω3-FFAs and 20 μM ATRA for 48 h. The expression of PARP protein.

    BOC-D-FMK purchased from MCE. Usage Cited in: Exp Cell Res. 2018 Sep 1;370(1):103-115.

    Representative Western blots show the change of truncated tau in cells transfected with wild type human tau, followed by treatment with 1 μM Stau for 10hr, and interference with Boc-D-FMK by addition 2 hr prior to the addition Stau.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Boc-D-FMK is a cell-permeable, irreversible and broad spectrum caspase inhibitor; inhibits apoptosis stimulated by TNF-α with an IC50 of 39 µM.

    IC50 & Target[1]

    Caspase

     

    In Vitro

    Apoptosis is a pathway of cell death orchestrated by a family of proteases called caspases. Boc-D-fmk inhibits TNFα-stimulated reactive oxygen species (ROS) generation. Boc-D-FMK inhibits apoptosis stimulated by TNF-α with an IC50 of 39 µM[1]. BocD-fmk at 50 µM prevents genistein-induced apoptosis of p815 cells. Confocal microscopy shows that the release of mitochondrial apoptotic factors is inhibited by BocD-fmk[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Boc-D-FMK-fmk effectively attenuates the hepatocyte apoptosis in bile duct-ligated rats and may improve the survival rates after endotoxin challenge[3]. A single injection of Boc-D-FMK results in longterm protection of MNs against root avulsion-induced death for more than 8 weeks and the Boc-D-FMK-treated MNs are able to regenerate their axons into an implanted PN graft and reinnervate the target muscle[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    263.26

    Formula

    C₁₁H₁₈FNO₅

    CAS No.

    634911-80-1

    SMILES

    O=C(CC(C(CF)=O)NC(OC(C)(C)C)=O)OC

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Pure form -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (379.85 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7985 mL 18.9926 mL 37.9853 mL
    5 mM 0.7597 mL 3.7985 mL 7.5971 mL
    10 mM 0.3799 mL 1.8993 mL 3.7985 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (9.50 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (9.50 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (9.50 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Animal Administration
    [3]

    Rats: Boc-D-FMK is dissolved in DMSO. Male Sprague-Dawley rats group 1 (OBBOC-D) undergo common bile duct ligation and simultaneously treatment with Boc-D-FMK. The first dose of Boc-D-FMK (1.5 mg/kg) is injected into the inferior vena cava immediately after bile duct ligation. Subsequent doses of Boc-DFMK (1.5 mg/kg twice daily) are given intraperitoneally on the first and second postoperative days. The last dose (1.5 mg/kg) is given on the morning of the third postoperative day[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: >95.0%

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    Keywords:

    BOC-D-FMKCaspaseInhibitorinhibitorinhibit

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    Product Name:
    BOC-D-FMK
    Cat. No.:
    HY-13229
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